Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissue

We describe steps for tissue processing, enzymatic digestion, Percoll density gradient separation, cell lysis, cell suspension quality control, and scRNA library construction. We then detail sequencing and data analysis. This protocol is applicable to preparing single-cell suspensions from other human liver tissue samples.PMID:37459235 | DOI:10.1016/j.xpro.2023.102449
Source: Cancer Control - Category: Cancer & Oncology Authors: Source Type: research