The evolution and functional divergence of 10 Apolipoprotein D ‐like genes in Nilaparvata lugens

In this study, we identified 10ApoD-like genes (NlApoD1 −10) with distinct spatiotemporal expression patterns inNilaparvata lugens (BPH), which is an important pest of rice.NlApoD1 −10 were found to be distributed on 3 chromosomes in a tandem array ofNlApoD1/2,NlApoD3 −5, andNlApoD7/8, and show sequence and gene structural divergence in the coding regions, indicating that multiple gene duplication events occurred during evolution. Phylogenetic analysis revealed that NlApoD1 −10 can be clustered into 5 clades, with NlApoD3−5 and NlApoD7/8 potentially evolving exclusively in the Delphacidae family. Functional screening using an RNA interference approach revealed that onlyNlApoD2 was essential for BPH development and survival, whereasNlApoD4/5 are highly expressed in testes, and might play roles in reproduction. Moreover, stress response analysis revealed thatNlApoD3 −5/9,NlApoD3 −5, andNlApoD9 were up-regulated after treatment with lipopolysaccharide, H2O2, and ultraviolet-C, respectively, indicating their potential roles in stress resistance.
Source: Insect Science - Category: Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research