Recombinant Endopeptidases IdeS and IdeZ and Their Potential Application

AbstractEndopeptidases IdeS and IdeZ (streptococcal virulence factors that specifically cleave IgG heavy chains) are of particular interest because of their potential use in biotechnology, medicine, and veterinary. Genes encoding these enzymes were cloned and expressed inEscherichia coli heterologous expression system (ideS was cloned from aStreptococcus pyogenes collection strain;ideZ fromStreptococcus zooepidemicus was synthesized). The 6His-tag was introduced into the amino acid sequence of each endopeptidase, and IdeS and IdeZ were purified by metal affinity chromatography to an apparent homogeneity (according to polyacrylamide gel electrophoresis). Purified enzymes were active against human and animal IgGs; their specificity toward human IgGs was confirmed by polyacrylamide gel electrophoresis. Recombinant IdeZ was used for immunological analysis of equine strangles infection (diagnostics and determination of the titer of specific antibodies in blood). Hence, IdeZ can be used in veterinary and sanitary microbiology to diagnose infections caused byStreptococcus  equi andS.  zooepidemicus in addition to its application in medicine and biotechnology.
Source: Biochemistry (Moscow) - Category: Biochemistry Source Type: research