Development of water-soluble AIE-based wash-free A[math] probes superior to commercial ThT

Journal of Innovative Optical Health Sciences, Ahead of Print. Alzheimer ’s disease (AD) is a typical neurodegenerative disease. [math]-amyloid (A[math] plaque is the most prominent pathological biomarker associated with the progression of AD. Conventional A[math] probes, including commercial probe ThT, usually suffer from tedious washing procedures. Herein, novel AIE- active A[math] probes with excellent water solubility, named DE-V1-PYC3 and DE-V1-PYOH, were developed for the detection and image of A[math] without tedious washing procedures. Compared with commercial probe ThT, the AIE-active A[math] probes exhibited better sensitivity and affinity to A[math] agg regates. Moreover, for ThT, the washing procedures are essential to obtain high signal-to-noise ratio (SNR) images of A[math] plaques in AD brain tissue slices. DE-V1-PYC3 and DE-V1-PYOH can label A[math] plaques in AD brain tissue slices with high SNR even without tedious washing procedures.
Source: Journal of Innovative Optical Health Sciences - Category: Biomedical Science Authors: Source Type: research