Long-Read Nanopore Sequencing of RPGR ORF15 is Enhanced Following DNase I Treatment of MinION Flow Cells

We report the novel finding that long-read nanopore sequencing can read throughRPGR-ORF15, a DNA sequence not captured by short-read next-generation sequencing (NGS), but with a more reduced yield. Use of a flow cell wash kit containing DNase I unblocks the pores, allowing reloading of further library aliquots over a 72-h period, increasing yield. The workflow we describe provides a novel solution to the need for a rapid, robust, scalable, cost-effectiveORF15 screening protocol.

http://link.springer.com/10.1007/s40291-023-00656-z

Source: Molecular Diagnosis and Therapy - June 7, 2023 Category: Molecular Biology Source Type: research