Response to Comment on Carrasco et  al. Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived β-Like Cells. Diabetes 2022;71:862–869

We thank Memon and Abdelalim (1) for their comments on our article (2). In short, they point out that the induced pluripotent stem cell (iPSC) lines that we used in our experiments contained mosaic trisomy 1 (47,XY,+1) in the mutated lines with the potential consequence that this trisomy would invalidate the study results. Memon and Abdelalim also point out that the genome-edited lines used as control lines did not contain this trisomy 1 (47,XY,+1), i.e., these gene-edited lines were 46,XY (with additional mosaicism, including deletions of chromosome 18) and hence did not represent appropriate controls. Furthermore, Memon and Abdelalim refer to an article by Hua et  al. (3) that was retracted (4) due to misrepresentation of karyotypic results. Memon and Abdelalim (1) conclude that “this signifies the importance of ensuring normal chromosomal profiles for [human pluripotent stem cell] lines used in the experiments.”
Source: Diabetes - Category: Endocrinology Source Type: research