Liquiritigenin, isoliquiritigenin rich extract of glycyrrhiza glabra roots attenuates inflammation in macrophages and collagen-induced arthritis in rats

AbstractLiquiritigenin (LTG) and its bioprecursor isoliquiritigenin(ISL), the main bioactives from roots of Glycyrrhiza genus are progressively documented as a potential pharmacological agent for the management of chronic diseases. The aim of this study was to evaluate the pharmacological potential of liquiritigenin, isoliquiritigenin rich extract ofGlycyrrhiza glabra roots (IVT-21) against the production of pro-inflammatory cytokines from activated macrophages as well as further validated the efficacy in collagen-induced arthritis model in rats. We also performed the safety profile ofIVT-21 using standardin-vitro andin-vivo assays. Results of this study revealed that the treatment ofIVT-21 and its major bioactives (LTG, ISL) was able to reduce the production of pro-inflammatory cytokines (TNF- α, IL-6) in LPS-activated primary peritoneal macrophages in a dose-dependent manner compared with vehicle-alone treated cells without any cytotoxic effect on macrophages.In-vivo efficacy profile against collagen-induced arthritis in Rats revealed that oral administration ofIVT-21 significantly reduced the arthritis index, arthritis score, inflammatory mediators level in serum.IVT-21 oral treatment is also able to reduce the NF кB-p65 expression as evidence of immunohistochemistry in knee joint tissue and mRNA level of pro-inflammatory cytokines in paw tissue in a dose-dependent manner when compared with vehicle treated rats. Acute oral toxicity profile ofIVT-21 demonstrated that it i...
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research