Epidemiological and diagnostic investigation on bovine theileriosis in Aswan Governorate, Egypt

AbstractThe present study was carried out to investigate the epidemiological and clinical status of bovine Theileriosis in Aswan governorate. During a 2-year study, 265 cattle were clinically suspected upon careful clinical examination asTheileria annulata (T. annulata) infected animals. Conventional diagnosis based on blood and lymph smears examinations showed that, the prevalence of Tropical Theileriosis in cattle in Aswan governorate was 56 (21.13%). Stained blood smears showed the presence of macro and/or micro-schizonts inside lymphocyte (Koch ’s blue bodies. Intraerythrocytic stages ofTheileria annulata piroplasms inside RBCs. Polymerase chain reactions ofT. annulata merozoite-piroplasms surface antigen Targeting gene: (Tams-1), revealed positive 29 (58%) animals confirmed by visualization of specific bands at 768  bp. Positive results could be detected in suspected cattle that showed positive or negative blood smear results that confirmed the high sensitivity of the PCR technique compared with the conventional method for diagnosis of bovine tropical Theileriosis. PCR proved to be a highly sensitive and accu rate method for diagnosis of bovine tropical theileriosis especially in the detection of samples that was negative on blood and lymph smears.
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research