Bioinformatic survey of CRISPR loci across 15 Serratia species

This study has contributed to extending the general knowledge of the CRISPR –Cas systems, particularly in Serratia, a ubiquitous genus detected in different environments. AbstractThe Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins (CRISPR –Cas) system of prokaryotes is an adaptative immune defense mechanism to protect themselves from invading genetic elements (e.g., phages and plasmids). Studies that describe the genetic organization of these prokaryotic systems have mainly reported on the Enterobacteriaceae family (now reorganized within the order of Enterobacterales). For some genera, data on CRISPR–Cas systems remain poor, as in the case ofSerratia (now part of theYersiniaceae family) where data are limited to a few genomes of the speciesmarcescens. This study describes the detection, in silico, of CRISPR loci in 146Serratia complete genomes and 336 high-quality assemblies available for the speciesficaria,fonticola,grimesii,inhibens,liquefaciens,marcescens,nematodiphila,odorifera,oryzae,plymuthica,proteomaculans,quinivorans,rubidaea,symbiotica, andureilytica. Apart from subtypes I-E and I-F1 which had previously been identified inmarcescens, we report that of I-C and the I-E unique locus 1, I-E*, and I-F1 unique locus 1. Analysis of the genomic contexts for CRISPR loci revealedmdtN-phnP as the region mostly shared (grimesii,inhibens,marcescens,nematodiphila,plymuthica,rubidaea, andSerratia sp.). Three new contexts detected in ...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research