Isolation and characterization of N ‐(2‐Hydroxyethyl)hexadecanamide from Colletotrichum gloeosporioides with apoptosis‐inducing potential in breast cancer cells

Schematic presentation of mechanism of anticancer activity ofN-(2-Hydroxyethyl)hexadecanamide (Palmitoylethanolamide; PEA) against human breast cancer cells. Antioxidant activity-guided fractionation and various spectroscopy techniques identify bioactive fraction 1 asN-(2-Hydroxyethyl)hexadecanamide (PEA).In-Silico study revealed that PEA shows potential binding affinity with the apoptotic inducer proteins Bax, Bcl-2, P21, and P53. Further,in vitro study validated theIn-Silico analysis and showed that PEA induces apoptosis in human breast cancer cells, MDA-MB-231 and MCF-7 by altering the expression level of extrinsic and intrinsic pathways related genes. AbstractEndophytic fungi are a well-established reservoir of bioactive compounds that are pharmaceutically valuable and therefore, contribute significantly to the biomedical field. The present study aims to identify the bioactive anticancer compound from ethyl acetate extract of fungal endophyte,Colletotrichum gloeosporioides associated with the leaf of the medicinal plantOroxylum indicum. The fatty acid amide compoundN-(2-Hydroxyethyl)hexadecanamide (Palmitoylethanolamide; PEA) was identified using antioxidant activity-guided fractionation assisted with tandem liquid chromatography coupled with quadrupole time of flight mass spectrometry, Fourier transform-infrared spectroscopy, time-of-flight mass spectrometry, and nuclear magnetic resonance.In-Silico molecular docking analysis showed that PEA potentially docked to the act...
Source: BioFactors - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research