Levofloxacin induces differential effects in the transcriptome between the gut, peripheral and axial joints in the Spondyloarthritis DBA/1 mice: Improvement of intestinal dysbiosis and the overall inflammatory process

by Susana Aide é González-Chávez, Joan S. Salas-Leiva, Dayana E. Salas-Leiva, Salma Marcela López-Loeza, Jasanai Sausameda-García, Erasmo Orrantia-Borunda, Rubén Burgos-Vargas, Maria Fernanda Alvarado-Jáquez, Mayra Torres-Quintana, Rubén Cuevas-Martínez, Eduardo Chaparro-Barrera, Carlos Marín-Terrazas, G erardo Pável Espino-Solís, José Pablo Romero-López, Brian de Jesús Bernal-Alferes, César Pacheco-Tena To analyze the effect of levofloxacin-induced intestinal microbiota modifications on intestinal, joint, and systemic inflammation in the DBA/1 mice with spontaneous arthritis. The study included two groups of mice, one of which received levofloxacin. The composition and structure of the microbiota were determined in the mice’s stool using 16S rRNA sequencing; the differential taxa and metabolic pathway between mice treated with levofloxacin and control mice were also defied. The effect of levofloxacin was evaluated in the intestines, hind paws, and spines of mice through DNA microarray tra nscriptome and histopathological analyses; systemic inflammation was measured by flow cytometry. Levofloxacin decreased the pro-inflammatory bacteria, including Prevotellaceae, Odoribacter, and Blautia, and increased the anti-inflammatory Muribaculaceae in mice’s stool. Histological analysis confi rmed the intestinal inflammation in control mice, while in levofloxacin-treated mice, inflammation was reduced; in the hind paws and spines, levofloxacin also decreased the in...
Source: PLoS One - Category: Biomedical Science Authors: Source Type: research