Natural monoterpenes-laden electrospun fibrous scaffolds for endodontic infection eradication

AbstractThis investigation aimed to synthesize poly(d,l-lactide) (PLA)-based fibrous scaffolds containing natural essential oils (i.e., linalool and citral) and determine their antimicrobial properties and cytocompatibility as a clinically viable cell-friendly disinfection strategy for regenerative endodontics. PLA-based fibrous scaffolds were fabricated via electrospinning with different concentrations of linalool and citral. The micromorphology and average diameter of the fibers was investigated through scanning electron microscopy (SEM). The chemical composition of the scaffolds was inferred by Fourier-transform infrared spectroscopy (FTIR). Antimicrobial efficacy againstEnterococcus faecalis andActinomyces naeslundii was also evaluated by agar diffusion and colony-forming units (CFU) assays. The scaffolds ’ cytocompatibility was determined using dental pulp stem cells (DPSCs). Statistical analyses were performed and the significance level was set atα = 5%. Linalool and citral’s incorporation in the PLA fibrous scaffolds was confirmed in the FTIR spectra. SEM images indicate no morphological changes upon inclusion of the essential oils, except the reduced diameter of 40% linalool-laden fibers (p <  0.05). Importantly, significant antimicrobial properties were reported for citral-containing scaffolds for CFU/mL counts (p <  0.05), while only 20% and 40% linalool-laden scaffolds reduced CFU/mL (p <  0.05). Meanwhile, the inhibition halos were ve...
Source: Odontology - Category: Dentistry Source Type: research