GSE214776 Endothelial deletion of the cytochrome P450 reductase leads to cardiac remodelling

Contributors : Melina Lopez ; Pedro F Malacarne ; Deepak P Ramanujam ; Timothy Warwick ; Niklas M üller ; Jiong Hu ; Matthias Dewenter ; Andreas Weigert ; Stefan Günther ; Ralf Gilsbach ; Stefan Engelhardt ; Ralf P Brandes ; Flávia RezendeSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe cytochrome P450 reductase (POR) transfers electrons to all microsomal cytochrome P450 enzymes (CYP450) thereby driving their activity. In the vascular system, the POR/CYP450 system has been linked to the production of epoxyeicosatrienoic acids (EETs) but also to the generation of reactive oxygen species. In cardiac myocytes (CMs), EETs have been shown to modulate the cardiac function and have cardioprotective effects. The functional importance of the endothelial POR/CYP450 system in the heart is unclear and was studied here using endothelial cell-specific, inducible knockout mice of POR (ecPOR-/-). RNA sequencing of murine cardiac cells revealed a cell type-specific expression of different CYP450 homologues. Cardiac endothelial cells mainly expressed members of the CYP2 family which produces EETs, and of the CYP4 family that generates omega fatty acids. Tamoxifen-induced endothelial deletion of POR in mice led to cardiac remodelling under basal conditions, as shown by an increase in heart weight to body weight ratio and an increased CM area as compared to control animals. Endothelial deletion of POR was associated with a significant increa...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research