Characterization of Leuconostoc mesenteroides MJM60376 as an oral probiotic and its antibiofilm activity

In this study, 67 lactic acid bacteria (LAB) were isolated from fermented food and screened for antagonistic activity againstStreptococcus mutans, the causative pathogen of dental caries.Leuconostoc mesenteroides MJM60376 showed the highest antagonistic activity againstS. mutans KCTC3065.L. mesenteroides MJM60376 also showed oral probiotic characteristics including weak acid production, lysozyme tolerance, adhesion to oral epithelial cell (YD-38), antibiotic susceptibility, and good coaggregation ability withS. mutans. Furthermore, the biofilm formation ofS. mutans was significantly reduced when cocultured withL. mesenteroides. Scanning electron microscopy analysis showed that amounts of attached bacteria ofS. mutans and network-like structures were significantly reduced byL. mesenteroides MJM60376. Cell-free supernatant (CFS) ofL. mesenteroides MJM60376 also greatly inhibited biofilm formation ofS. mutans from the adherent stage, the activity remained even after it was treated with catalase, trypsin, or pH neutralized. Expression levels of biofilm formation-related genes were significantly reduced inS. mutans when it was treated with the CFS ofL. mesenteroides MJM60376. Therefore,L. mesenteroides MJM60376 has great potential to be used as a multifunctional ingredient.
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research