Identification of individuals by RNA sequencing of low template samples

In this pilot study, we made dilutions of DNA/RNA co-extractions (1,000-12.5pg gDNA) and whole transcriptome shotgun sequenced RNA on a NovaSeq 6000 platform (Illumina). Whole blood samples from two individuals were sequenced in triplicate for each dilution.More than 450,000 RNA variants were identified to have overlapping positions with the gnomAD 3.0 DNA variant database in the 50 genes with most reads. Between 7,000 and 45,000 of these loci were covered with at least 70 reads in the various dilutions.

Source: Forensic Science International: Genetics Supplement Series - October 25, 2022 Category: Forensic Medicine Authors: Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Claus B ørsting Source Type: research