Knockdown of Endogenous Nucb2/Nesfatin-1 in the PVN Leads to Obese-Like Phenotype and Abolishes the Metformin- and Stress-Induced Thermogenic Response in Rats
Horm Metab Res DOI: 10.1055/a-1926-7280Nesfatin-1, the cleavage product of nucleobindin-2, is an anorexigenic peptide
and major regulator of energy homeostasis. Beyond reducing food intake and
increasing energy expenditure, it is also involved in regulating the stress
response. Interaction of nucleobindin-2/nesfatin-1 and glucose
homeostasis has been observed and recent findings suggest a link between the
action of the antidiabetic drug metformin and the nesfatinergic system. Hence,
this study aimed to clarify the role of nucleobindin-2/nesfatin-1 in the
paraventricular nucleus of the hypothalamus in energy homeostasis as well as its
involvement in stress- and metformin-mediated changes in energy expenditure.
Knockdown of nucleobindin-2/nesfatin-1 in male Wistar rats led to
significantly increased food intake, body weight, and reduced energy expenditure
compared to controls. Nucleobindin-2/nesfatin-1 knockdown animals
developed an obese-like phenotype represented by significantly increased fat
mass and overall increase of circulating lipids. Concomitantly, expression of
nucleobindin-2 and melanocortin receptor type 3 and 4 mRNA in the
paraventricular nucleus was decreased indicating successful knockdown and
impairment at the level of the melanocortin system. Additionally, stress induced
activation of intersca...
Source: Hormone and Metabolic Research - Category: Endocrinology Authors: Stephan, Daniel Taege, Natalie Dore, Riccardo Folberth, Julica J öhren, Olaf Schwaninger, Markus Lehnert, Hendrik Schulz, Carla Tags: Original Article: Endocrine Research Source Type: research
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