The detection and phylogenetic analysis of Anaplasma phagocytophilum-like 1, A. ovis and A. capra in sheep: A. capra divides into two genogroups

In this study, the presence, prevalence, and genotypes ofAnaplasma phagocytophilum, A. ovis, andA. capra in sheep were investigated based on 16  S SSU rRNA,groEL, andgtlA gene-specific polymerase chain reaction (PCR), respectively. The sequences of the genes were used for detection of the phylogenetic position of the species. Additionally, a restriction fragment length polymorphism (RFLP) were carried out for discrimination ofA. phagocytophilum and related variants (A. phagocytophilum-like 1 and 2). The prevalence ofAnaplasma spp. was found as 25.8% (101/391), while it was found thatA.ovis,A. phagocytophilum-like 1, andA. capra are circulating in the sheep herds in Kyrgyzstan, according to the PCRs, RFLP and the partial DNA sequencing results. The positivity rates ofA. phagocytophilum-like 1,A. ovis, andA. capra genotype-1 were 6.9, 22.5, and 5.3%, respectively. A total of 32 (8.2%) sheep were found to be mix infected. Moreover, phylogenetic analyses and sequence comparison with those available in the GenBank showed thatA. capra formed two distinct genetic groups (A. capra genotype-1 andA. capra genotype-2). Considering the zoonotic potential of these species, it may be necessary to make changes in the interpretation of anaplasmosis cases in animals and there is a need for further studies to determine the pathogenicity of the species/genotypes circulating in animals.
Source: Veterinary Research Communications - Category: Veterinary Research Source Type: research