Comparative analysis of Listeria monocytogenes plasmid transcriptomes reveals common and plasmid ‐specific gene expression patterns and high expression of noncoding RNAs

This study performed gene expression analysis of threeListeria monocytogenes plasmids from three differentL. monocytogenes strains. These findings further our understanding of the dynamic expression ofL. monocytogenes plasmid-encoded genes in diverse environmental conditions and highlight the need to expand the study ofL. monocytogenes plasmid gene function AbstractRecent research demonstrated that someListeria monocytogenes plasmids contribute to stress survival. However, only a few studies have analyzed gene expression patterns ofL. monocytogenes plasmids. In this study, we identified four previously published stress-response-associated transcriptomic data sets which studied plasmid-harboringL. monocytogenes strains but did not include an analysis of the plasmid transcriptomes. The four transcriptome data sets encompass three distinct plasmids from three differentL. monocytogenes strains. Differential gene expression analysis of these plasmids revealed that the number of differentially expressed (DE)L. monocytogenes plasmid genes ranged from 30 to 45 with log2 fold changes of −2.2 to 6.8, depending on the plasmid. Genes often found to be DE included the cadmium resistance genescadA andcadC, a gene encoding a putative NADH peroxidase, the putative ultraviolet resistance geneuvrX, and several uncharacterized noncoding RNAs (ncRNAs). Plasmid-encoded ncRNAs were consistently among the highest expressed genes. In addition, one of the data sets utilized the same experimental co...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research