Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer

Nature Protocols, Published online: 19 August 2022; doi:10.1038/s41596-022-00729-zIdentifying metabolites in cells of the tumor microenvironment is an area of intense study. This protocol describes specimen collection, enrichment of human immune cell populations and analysis by flow cytometry and LC–MS.

Source: Nature Protocols - August 19, 2022 Category: Biomedical Science Authors: Marisa K. Kilgour Sarah MacPherson Lauren G. Zacharias Jodi LeBlanc Sindy Babinszky Gabrielle Kowalchuk Scott Parks Ryan D. Sheldon Russell G. Jones Ralph J. DeBerardinis Phineas T. Hamilton Peter H. Watson Julian J. Lum Source Type: research