Optimization of bovine embryonic fibroblast feeder layer prepared by Mitomycin C

In conclusion, the bEF-feeder prepared with 14 μg/mL Mitomycin C for 3 h or 16 μg/mL Mitomycin C for 3 h could effectively maintains the growth of bESCs, and bEF-feeder is more suitable for bESCs culture than the MEF-feeder.
Source: Cell and Tissue Banking - Category: Stem Cells Source Type: research