E-192 Association of myeloperoxidase-DNA complexes and high mobility group box 1 protein with delayed cerebral ischemia following aneurysmal subarachnoid hemorrhage

High mobility group box 1 protein (HMGB1) serves as a key player in aseptic inflammation. Admission serum HMGB1 has been identified as a biomarker predictive of delayed cerebral ischemia following aneurysmal subarachnoid hemorrhage (aSAH). Released by leucocytes and platelets, HMGB1 induces neutrophils to release condensed chromatin and protein granules, termed neutrophil extracellular traps (NETs). Myeloperoxidase (MPO)-DNA complexes are one major NET biomarker and have been associated with arterial and venous thrombosis formation. The prevalence and role of MPO-DNA in aSAH remains to be determined.A post-hoc analysis of a prospective, blinded, single-center biomarker observational study to investigate the role of HMGB1 was performed to explore if MPO-DNA complexes can be identified in aSAH patients, and if so, whether their titers are associated with DCI defined as new infarction on CT-scan not adjudicated to treatment. Secondary analysis was performed to explore association with clinical vasospasm. Admission and day 4 serum samples were analyzed for MPO-DNA complexes.One hundred consecutive non-traumatic spontaneous SAH patients were enrolled and 83 revealed an aneurysm on angiography. Five patients (5/83) died <48h not allowing for DCI determination per definition. MPO-DNA complexes were detected in all serum samples. HMGB1 level significantly correlated with MPO-DNA levels on admission as well as day 4 (p<0.001, r=0.606 and p<0.001, r=0.622, respectively).In 29/...
Source: Journal of NeuroInterventional Surgery - Category: Neurosurgery Authors: Tags: SNIS 19th annual meeting electronic poster abstracts Source Type: research