Macrophage Depletion Attenuates Acute Renal Damage after Exhaustive Exercise in Mice
Int J Sports Med DOI: 10.1055/a-1827-3261Exhaustive exercise is known to induce acute renal damage. However, the precise
mechanisms remain unclear. We investigated the effects of macrophage depletion
on exhaustive exercise-induced acute renal damage. Male
C57BL/6 J mice were divided into four groups: sedentary with
control liposome (n=8), sedentary with clodronate liposome
(n=8), exhaustive exercise with control liposome (n=8), and
exhaustive exercise with clodronate liposome (n=8). Mice were treated
with clodronate liposomes or control liposomes intraperitoneally for
48 h before undergoing exhaustive exercise. Renal function and renal
histology were tested at 24 h. The expression levels of kidney injury
molecule (KIM)-1 and inflammatory cytokines in kidney tissues were measured by
quantitative RT-PCR, and KIM-1 concentration was semi-quantified by
immunostaining. As a result, exhaustive exercise increased macrophage
infiltration into the kidney. However, clodronate reduced it. Although
exhaustive exercise resulted in an increase in KIM-1 mRNA expression levels and
concentration, injection of clodronate liposome reduced it. In addition, TUNEL
positive apoptotic cells were increased after exercise, but significantly
reduced by clodronate. Clodronate liposome treatment also decreased the mRNA
ex...
Source: International Journal of Sports Medicine - Category: Sports Medicine Authors: Mizokami, Tsubasa Shimada, Michiko Suzuki, Katsuhiko Tags: Genetics and Molecular Biology Source Type: research