The biosynthesis of lipoic acid: a saga of death, destruction, and rebirth
NIH Director's Wednesday Afternoon Lecture Series
Research in the Booker Lab focuses on understanding how Nature harnesses the power of radicals to effect kinetically challenging enzymatic reactions, many of which are critical to proper cellular functioning. Much of the lab ’ s work has centered around the enzymatic use of S-adenosylmethionine and iron-sulfur clusters to generate a 5 ’ -deoxyadenosyl 5 ’ -radical, used as a key intermediate by members of the radical S-adenosylmethionine (SAM) superfamily to catalyze over 60 different reaction types. One major interest of the Booker Lab is the use of radical SAM chemi...
Source: Videocast - All Events - April 24, 2019 Category: General Medicine Tags: Upcoming Events Source Type: video
Detection of Dichlorvos Adducts on Proteins from a Liver-derived Cell Line
Proteomics Interest Group
The toxicity of dichlorvos (DDVP), an organophosphate (OP) pesticide, classically results from modification of the serine in the active sites of cholinesterases. However, DDVP also forms adducts on unrelated targets such as transferrin and albumin, suggesting that DDVP could cause perturbations in cellular processes by modifying non-cholinesterase targets. Here, we identify novel DDVP-modified targets in lysed human hepatocyte-like cells (HepaRG) using a direct liquid chromatography-mass spectrometry (LC-MS) assay of cell lysates incubated with DDVP or using a competitive pull-down experiments wi...
Source: Videocast - All Events - February 27, 2014 Category: Journals (General) Tags: Upcoming Events Source Type: video
