Detection of Dichlorvos Adducts on Proteins from a Liver-derived Cell Line
Proteomics Interest Group The toxicity of dichlorvos (DDVP), an organophosphate (OP) pesticide, classically results from modification of the serine in the active sites of cholinesterases. However, DDVP also forms adducts on unrelated targets such as transferrin and albumin, suggesting that DDVP could cause perturbations in cellular processes by modifying non-cholinesterase targets. Here, we identify novel DDVP-modified targets in lysed human hepatocyte-like cells (HepaRG) using a direct liquid chromatography-mass spectrometry (LC-MS) assay of cell lysates incubated with DDVP or using a competitive pull-down experiments with a biotin-linked organophosphorus compound (10-fluoroethoxyphosphinyl-N-biotinamidopentyldecanamide; FP-biotin), which competes with DDVP for similar binding sites. We show that DDVP forms adducts to several proteins important for the cellular metabolic pathways and differentiation, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin. We validated the results using purified proteins and enzymatic assays. The study not only identified novel DDVP-modified targets but also suggested that the modification directly inhibits the enzymes. The current approach provides information for future hypothesis-based studies to understand the underlying mechanism of toxicity of DDVP in non-neuronal tissues.For more information go to http://proteome.nih.govAir date: 3/6/2014 10:00:00 AM
Esene et al,1 in their brilliantly conceived and presented manuscript, “Misclassification of Case Control Studies in Neurosurgery and Proposed Solutions,” raise very good and valid points about the misclassification of published research manuscripts and potential harm done by the mislabeling or misrepr esentation of manuscript content. They eloquently demonstrate that, although many studies have been published with the inclusion of “case control study” in the title or methodology to describe the study design, often such studies are not “case control studies” but rather “cohor...
Controversy exists regarding which treatment option (full endoscopic vascular decompression or endoscopic-assisted vascular decompression) is better in the treatment of trigeminal neuralgia (TN). One group suggests that the most of the procedure should be done under a microscope and that endoscopic assistance should be undertaken to detect vessels placed anterior to the nerve and to confirm that the decompression has been performed properly. Other group believes that the whole procedure can be done safely with the use of a full endoscopic technique.
Evaluating alternatives to the invasive pterional craniotomy is critical, as it will help practitioners make safe and effective clinical decisions. We read the review1 published in a recent issue of WORLD NEUROSURGERY. However, Rychen et al's results are questionable due to several methodological issues listed as follows.
We studied with keen interest the article by Wang et al1 regarding clinical behavior and prognosis of gliomas in human immunodeficiency virus (HIV) patients.
We would like to thank Dr. Yang and his or her colleagues for their excellent and deep review of our article. In their letter to the editor,1 they made several recommendations for our article.
I read with great interest the article by Dubey et al.1 They reported a successful result of a large number of patients with trigeminal neuralgia and technical tips. I myself use endoscope for assistance to observe the dead corner behind the suprameatal tubercle and near the Meckel cave, and the panoramic view that the endoscope provides is useful for thorough observation of the cisternal portion of the trigeminal nerve. However, I am doubtful about the usefulness of totally endoscopic microvascular decompression (MVD) for several reasons.
In the recently published article by Loewenstern et al,1 the authors attempted to determine the utility of optical coherence tomography (OCT) in the diagnosis and management of compressive optic neuropathy secondary to fibrous dysplasia (FD). OCT is a relatively new imaging modality, which uses high-resolution cross-sections of the retina to determ ine the retinal nerve fiber layer (RNFL) thickness. The study evaluated 6 patients with cranial base FD for optic nerve compression and RNFL thinning, using computed tomography (CT) and OCT, respectively.
We read with great interest the recent study by Zhu et al1 entitled “Rupture Risk of Cerebral Arteriovenous Malformations During Pregnancy and Puerperium: A Single-Center Experience and Pooled Data Analysis.” The authors performed a retrospective study to compare risk of rupture in pregnant and nonpregnant female patients with arteriovenous malfor mation (AVM) and assess current evidence regarding rupture risk of AVM during pregnancy and puerperium by pooled data analysis. They concluded that an increase in annual rate of cerebral AVM hemorrhage during pregnancy and puerperium was found.