Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies
The transcriptome has shown tremendous potential for the comprehensive investigation of gene expression profiles and transcriptional levels in comparative biology, the identification of regulatory mechanism of transcriptional regulators, and the evaluation of target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. The traditional microarray and advanced next-generation RNA sequencing technologies (RNA-seq) provide powerful and effective tools for the determination of the transcriptome of bacterial cells. In this chapter, we provide a detailed protocol for scientists who want to investigate ...
Source: Springer protocols feed by Microbiology - October 6, 2013 Category: Microbiology Source Type: news
Nutrition 101: Biotin
(HealthCastle.com) Biotin is one of 8 B vitamins. All of the B vitamins are water soluble, meaning that the body does not store them but excretes them in the urine.
Recommended Intakes
The Dietary Reference Intakes (DRI) for biotin are shown below:
read more (Source: HealthCastle.com Nutrition Tips - written by Registered Dietitians)
Source: HealthCastle.com Nutrition Tips - written by Registered Dietitians - September 30, 2013 Category: Nutrition Source Type: news
Proud Distributor of RayBiotech's Antibody Arrays and ELISAs
Pioneer and Market LeaderI am pleased to announce we are a distribution partner for RayBiotech. The solutions we offer include Antibody Arrays and ELISA Kits. With these additions, you now have many options for the either the qualitative, semi-quantitative or quantitative measurement of protein expression. From single antibody pair ELISAs to multiplexed arrays. The arrays enable you to simultaneously analyze up to 80 cytokine, inflammatory response, growth factor or angiogenesis related proteins.In this posting, I focus on our new C-Series Antibody Array Kits. The kits utilize the sandwich immunoassay principle, wherein a ...
Source: Neuromics - August 20, 2013 Category: Neuroscience Tags: sandwich ELISA Cytokine signaling Cytokine Antibody Arrays Inflammatory Response Antibody Arrays Angiogenesis Antibody Arrays. Quantibody Arrays C-Series Antibody Arrays Source Type: news
Potential New Antibiotic Operates In A Completely Different Way
University of Adelaide research has produced a potential new antibiotic which could help in the battle against bacterial resistance to antibiotics. The potential new antibiotic targets a bacterial enzyme critical to metabolic processes. The compound is a protein inhibitor which binds to the enzyme (called biotin protein ligase), stopping its action and interrupting the life cycle of the bacteria... (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - July 5, 2013 Category: Consumer Health News Tags: MRSA / Drug Resistance Source Type: news
Detection of Cell Surface Dopamine Receptors
Dopamine receptors are a class of metabotropic G protein-coupled receptors. Plasma membrane expression is a key determinant of receptor signaling, and one that is regulated both by extra and intracellular cues. Abnormal dopamine receptor signaling is implicated in several neuropsychiatric disorders, including schizophrenia and attention deficit hyperactivity disorder, as well as drug abuse. Here, we describe in detail the application of two complementary applications of protein biotinylation and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surf...
Source: Springer protocols feed by Neuroscience - January 11, 2013 Category: Neuroscience Source Type: news
