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RIPK1 inhibition contributes to lysosomal membrane stabilization in ischemic astrocytes via a lysosomal Hsp70.1B-dependent mechanism
In this study, we investigated the molecular mechanisms underlying RIPK1-mediated astrocyte injury in vitro and in vivo. Primary cultured astrocytes were transfected with lentiviruses and then subjected to oxygen and glucose deprivation (OGD). In a rat model of permanent middle cerebral artery occlusion (pMCAO), lentiviruses carrying shRNA targeting RIPK1 or shRNA targeting heat shock protein 70.1B (Hsp70.1B) were injected into the lateral ventricles 5 days before pMCAO was established. We showed that RIPK1 knockdown protected against OGD-induced astrocyte damage, blocked the OGD-mediated increase in lysosomal membrane per...
Source: Acta Pharmacologica Sinica - April 13, 2023 Category: Drugs & Pharmacology Authors: Hua-Ping Du Yi Guo Yong-Ming Zhu De-Fei Gao Bo Lin Yuan Liu Yuan Xu Ali Said Taous Khan Li-Jun Liu Jian-Jun Zhu Yong Ni Hui-Ling Zhang Source Type: research

The lncRNA Malat1 functions as a ceRNA to contribute to berberine-mediated inhibition of HMGB1 by sponging miR-181c-5p in poststroke inflammation.
In this study, we explored the role of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (Malat1) in the process of BBR-induced inhibition of HMGB1 in ischemic brain. Before the 60-min MCAO surgery, the mice were pretreated with BBR (50 mg· kg-1 per day, ig) for 14 days or ICV injected with specific lentiviral vector or shRNA. We showed that MCAO caused marked increase in the expression Malat1 and HMGB1 in the ipsilateral cortex, which was significantly attenuated by pretreatment with BBR. Knockdown of Malat1 attenuated the inflammatory injury after brain ischemia, whereas overexpression of Malat1 exacerba...
Source: Acta Pharmacologica Sinica - August 19, 2019 Category: Drugs & Pharmacology Authors: Cao DW, Liu MM, Duan R, Tao YF, Zhou JS, Fang WR, Zhu JR, Niu L, Sun JG Tags: Acta Pharmacol Sin Source Type: research

Berberine attenuates ischemia-reperfusion injury through inhibiting HMGB1 release and NF- κB nuclear translocation.
In this study, we explored the mechanisms underlying the neuroprotective action of berberine in mice subjected transient middle cerebral artery occlusion (tMCAO). Male mice were administered berberine (25, 50 mg/kg/d, intragastric; i.g.), glycyrrhizin (50 mg/kg/d, intraperitoneal), or berberine (50 mg/kg/d, i.g.) plus glycyrrhizin (50 mg/kg/d, intraperitoneal) for 14 consecutive days before tMCAO. The neurological deficit scores were evaluated at 24 h after tMCAO, and then the mice were killed to obtain the brain samples. We showed that pretreatment with berberine dose-dependently decreased the infarct size, neur...
Source: Acta Pharmacologica Sinica - September 28, 2018 Category: Drugs & Pharmacology Authors: Zhu JR, Lu HD, Guo C, Fang WR, Zhao HD, Zhou JS, Wang F, Zhao YL, Li YM, Zhang YD, Yang CQ, Sun JG Tags: Acta Pharmacol Sin Source Type: research

Naloxone attenuates ischemic brain injury in rats through suppressing the NIK/IKK α/NF-κB and neuronal apoptotic pathways.
Naloxone attenuates ischemic brain injury in rats through suppressing the NIK/IKKα/NF-κB and neuronal apoptotic pathways. Acta Pharmacol Sin. 2018 Jun 14;: Authors: Wang X, Sun ZJ, Wu JL, Quan WQ, Xiao WD, Chew H, Jiang CM, Li D Abstract Although naloxone has been documented to exert neuroprotection in animal model of cerebral ischemia, the mechanism is not well understood. In this present study we investigated whether naloxone affected the mitochondrial apoptotic pathway in ischemic brain injury of rats. SD rats were subjected to a permanent middle cerebral artery occlusion surgery, and received na...
Source: Acta Pharmacologica Sinica - June 14, 2018 Category: Drugs & Pharmacology Authors: Wang X, Sun ZJ, Wu JL, Quan WQ, Xiao WD, Chew H, Jiang CM, Li D Tags: Acta Pharmacol Sin Source Type: research

Diterpene ginkgolides protect against cerebral ischemia/reperfusion damage in rats by activating Nrf2 and CREB through PI3K/Akt signaling.
This study was designed to investigate the neuroprotective effects of DGMI components against ischemic stroke in vivo and in vitro. Acute cerebral ischemic injury was induced in rats by occlusion of the middle cerebral artery (MCA) for 1.5 h followed by 24 h reperfusion. The rats were treated with DGMI (1, 3 and 10 mg/kg, iv) at the onset of reperfusion and 12 h after reperfusion. Administration of DGMI significantly decreased rat neurological deficit scores, reduced brain infarct volume, and induced protein kinase B (Akt) phosphorylation, which prompted the nuclear translocation of nuclear factor-erythroid 2-related facto...
Source: Acta Pharmacologica Sinica - March 15, 2018 Category: Drugs & Pharmacology Authors: Zhang W, Song JK, Yan R, Li L, Xiao ZY, Zhou WX, Wang ZZ, Xiao W, Du GH Tags: Acta Pharmacol Sin Source Type: research

Connexin 43 stabilizes astrocytes in a stroke-like milieu to facilitate neuronal recovery.
CONCLUSION: Cx43 can stabilize astrocytes and facilitate the resistance to the deleterious effects of a stroke-like milieu and promote neuronal recovery. PMID: 26095039 [PubMed - as supplied by publisher]
Source: Acta Pharmacologica Sinica - June 22, 2015 Category: Drugs & Pharmacology Authors: Wu LY, Yu XL, Feng LY Tags: Acta Pharmacol Sin Source Type: research