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Specialty: Biotechnology
Nutrition: Fish
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Total 3 results found since Jan 2013.
Molecular Characterization of U6 Promoters from Orange-Spotted Grouper (Epinephelus coioides) and Its Application in DNA Vector-Based RNAi Technology
Mar Biotechnol (NY). 2023 May 8. doi: 10.1007/s10126-023-10212-9. Online ahead of print.ABSTRACTThe U6 promoter, a typical RNA polymerase III promoter, is widely used to transcribe small RNAs in vector-based siRNA systems. The RNAi efficiency is mainly dependent on the transcriptional activity of the U6 promoter. However, studies have found that U6 promoters isolated from some fishes do not work well in distantly related species. To isolate a U6 promoter with high transcriptional efficiency from fish, in this study, we cloned five U6 promoters in orange-spotted grouper, of which only the grouper U6-1 (GU6-1) promoter conta...
Source: Marine Biotechnology - May 8, 2023 Category: Biotechnology Authors: Fengying Yan Xinxun Xiao Chen Long Lin Tang Chongwei Wang Mingqing Zhang Jin Zhang Haoran Lin Hai Huang Yong Zhang Shuisheng Li Source Type: research
