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Hepatitis B virus DNA polymerase displays an anti-apoptotic effect by interacting with elongation factor 1-alpha2 in hepatoma cells.
In this study, we aimed at investigating the impact of HBV Pol on host cellular processes, mainly apoptosis, and the underlying mechanisms. We showed a marked reduction in apoptotic rates in the HBV Pol-expressed HepG2 cells compared to controls. Moreover, a series of assays-i.e. yeast two-hybrid, GST pull-down, co-immunoprecipitation, and confocal laser scanning microscopy-identified the host factor eEF1A2 to be associated with HBV Pol. Furthermore, knockdown of the eEF1A2 gene by siRNA abrogated the HBV Pol-mediated anti-apoptotic effect with apoptosis induced by endoplasmatic reticulum (ER) stress inducer thapsigargin (...
Source: Journal of Microbiology and Biotechnology - October 23, 2020 Category: Biotechnology Authors: Niu X, Nong S, Gong J, Zhang X, Tang H, Zhou T, Li W Tags: J Microbiol Biotechnol Source Type: research

Silencing of the scavenger receptor (Class B - Type 1) gene using siRNA-loaded chitosan nanaoparticles in a HepG2 cell model.
In this study, we investigated the use of chitosan nanoparticles as non-viral delivery carriers of siRNA. As a model target, we selected the scavenger receptor (SR-B1), due to its proposed involvement in hepatitis C virus (HCV) internalization. Low molecular weight (LMW) chitosan nanoparticles were prepared by simple ionic gelation using sodium tripolyphosphate (TPP) as a cross-linking agent; a fixed chitosan and TPP concentration of 0.1% was used, and a chitosan to TPP weight ratios of 3:1, 5:1, and 9:1 were investigated. Nanoparticle uptake efficiency was measured using FITC-labeled chitosan nanoparticles and silencing o...
Source: Colloids and Surfaces - November 3, 2014 Category: Biotechnology Authors: Farid MM, Hathout RM, Fawzy M, Abou-Aisha K Tags: Colloids Surf B Biointerfaces Source Type: research