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Possibility for double optimization of siRNA intracellular delivery efficiency and antibacterial activity: Structure screening of pH-sensitive triblock amphiphilic polycation micelles
Colloids Surf B Biointerfaces. 2021 Oct 26;209(Pt 2):112178. doi: 10.1016/j.colsurfb.2021.112178. Online ahead of print.ABSTRACTOptimal combination of hydrophobic-hydrophilic balance, proton buffering and electrostatic interaction is the key issue for designing polycations as efficient gene vectors and antibacterial agents. Herein, we screened a series of pH-sensitive quaternary ammonium-based amphiphilic triblock copolymers, mPEG2k-P(DPAa/DMAb)-PQAc (TDDE-x), which had different pKa values and proton buffering capacities. Significantly, we found that both the highest siRNA intracellular delivery efficiency and the stronge...
Source: Colloids and Surfaces - November 6, 2021 Category: Biotechnology Authors: Yufeng Zhang Yanliang Dong Junhui Zhou Wenjun Huang Yidi Wu Shuyue Zhao Yongli Shi Suping Bai Chunhui Li Yuanyu Huang Anjie Dong Source Type: research

siRNAs encapsulated in Recombinant Capsid protein derived from Dengue serotype 2 virus inhibits the four serotypes of the virus and proliferation of cancer cells
In this study we have evaluated the utility of the protein as an siRNA carrier for anti Dengue viral and anti cancer applications using cell culture systems. We show that target specific siRNAs delivered using the capsid protein inhibit infection by the four serotypes of Dengue virus and proliferation of two cancer cell lines. Our data confirm the potential of the capsid for anti Dengue viral and anti cancer RNAi applications. In addition, we have optimized a fermentation strategy to improve the yield of E. coli expressed D2C protein since the reported yield of E.coli expressed flaviviral capsid proteins are low.
Source: Journal of Biotechnology - November 13, 2014 Category: Biotechnology Source Type: research

Efficient and specific gene knockdown by small interfering RNAs produced in bacteria
Nature Biotechnology 31, 350 (2013). doi:10.1038/nbt.2537 Authors: Linfeng Huang, Jingmin Jin, Padraig Deighan, Evgeny Kiner, Larry McReynolds & Judy Lieberman Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells and may be used for therapeutic purposes to knock down genes implicated in disease. Thus far, most synthetic siRNAs have been produced by chemical synthesis. Here we present a method to produce highly potent siRNAs in Escherichia coli. This method relies on ectopic expression of p19, an siRNA-binding protein found in a plant RNA virus. Whe...
Source: Nature Biotechnology - March 10, 2013 Category: Biotechnology Authors: Linfeng HuangJingmin JinPadraig DeighanEvgeny KinerLarry McReynoldsJudy Lieberman Tags: Research Source Type: research