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Development of peptide-based ratiometric fluorescent probe for sensing heparan sulfate and heparin in aqueous solutions at physiological pH and quantitative detection of heparan sulfate in live cells
This study presents the first ratiometric fluorescent probe capable of quantitatively detecting HS levels in aqueous solutions and live cells. The unique properties of peptide-based probe 3 make it a valuable tool for studying HS biology and potentially for diagnostic applications in various biological systems.PMID:37595478 | DOI:10.1016/j.bios.2023.115595
Source: Biosensors and Bioelectronics - August 18, 2023 Category: Biotechnology Authors: Sumita Subedi Hyun Jung Hwang Donghee Kang Pramod Kumar Mehta Nayeon Kim Hyojin Park Jae-Seon Lee Keun-Hyeung Lee Source Type: research

An miRISC-initiated DNA nanomachine for monitoring MicroRNA activity in living cells
Biosens Bioelectron. 2022 Oct 29;220:114828. doi: 10.1016/j.bios.2022.114828. Online ahead of print.ABSTRACTMicroRNAs (miRNAs) play an important role in post-transcriptional regulation of gene expression. However, methods to accurately detect miRNA activity in living cells are still limited. Here we developed a DNA nanomachine initiated by a miRNA-induced silencing complex (miRISC) for imaging miRNA activity in living cells. miRISC-mediated RNA cleavage reaction activated the DNA nanomachine by the specific cleavage of an RNA strand on the machine, resulting in autonomous movement of the walking leg around the AuNP surface...
Source: Biosensors and Bioelectronics - November 3, 2022 Category: Biotechnology Authors: Jin-Yu Wang Hua-Dong Li Pei-Qiang Ma Ying Zhou Bin-Cheng Yin Bang-Ce Ye Source Type: research

Exosomes-mediated synthetic Dicer substrates delivery for intracellular Dicer imaging detection.
In this study, we design a fluorescent labeling Dicer substrate and effectively deliver it into cell by exosomes derived from the target parent cells for intracellular Dicer expression level monitor and gene therapy. Using pre-miRNA let-7a as a model, the Dicer substrates with two terminals labeled with fluorescent and quencher group respectively was obtained by T4 RNA mediated ligase reaction from two short RNA sequences. Then, the substrate was packaged into exosomes by electroporation and delivered to target cells for intracellular dicer imaging detection. After packaging substrates into exosomes with little immunogenic...
Source: Biosensors and Bioelectronics - February 1, 2020 Category: Biotechnology Authors: Dai W, Su L, Lu H, Dong H, Zhang X Tags: Biosens Bioelectron Source Type: research