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Role of SNARE Proteins in the Insertion of KCa3.1 in the Plasma Membrane of a Polarized Epithelium
Targeting proteins to a specific membrane is crucial for proper epithelial cell function. KCa3.1, a calcium-activated, intermediate-conductance potassium channel, is targeted to the basolateral membrane (BLM) in epithelial cells. Surprisingly, the mechanism of KCa3.1 membrane targeting is poorly understood. We previously reported that targeting of KCa3.1 to the BLM of epithelial cells is Myosin-Vc-, Rab1-and Rab8-dependent. Here, we examine the role of the SNARE proteins VAMP3, SNAP-23 and syntaxin 4 (STX-4) in the targeting of KCa3.1 to the BLM of Fischer rat thyroid (FRT) epithelial cells. We carried out immunoblot, siRN...
Source: Frontiers in Physiology - June 27, 2022 Category: Physiology Source Type: research

Stimulatory Role of SPAK Signaling in the Regulation of Large Conductance Ca2+-Activated Potassium (BK) Channel Protein Expression in Kidney
In this study, we investigated the effect of SPAK on renal BK protein expression in both HEK293 cells and mouse kidney. In HEK293 cells, siRNA-mediated knockdown of SPAK expression significantly reduced BK protein expression and increased ERK1/2 phosphorylation, whereas overexpression of SPAK significantly enhanced BK expression and decreased ERK1/2 phosphorylation in a dose-dependent manner. Knockdown of ERK1/2 prevented SPAK siRNA-mediated inhibition of BK expression. Similarly, pretreatment of HEK293 cells with either the lysosomal inhibitor bafilomycin A1 or the proteasomal inhibitor MG132 reversed the inhibitory effec...
Source: Frontiers in Physiology - July 1, 2020 Category: Physiology Source Type: research

Interfering RNA against PKC- α Inhibits TNF-α-induced IP3R1 Expression and Improves Glomerular Filtration Rate in Rats with Fulminant Hepatic Failure.
CONCLUSION: RNAi targeting PKC-α inhibited TNF-α-induced IP3R1 overexpression, and in turn improved compromised GFR in the development of acute kidney injury during FHF in rats. PMID: 29357415 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Renal Physiology - January 10, 2018 Category: Physiology Authors: Wang DL, Dai WY, Wang W, Wen Y, Zhou Y, Zhao YT, Wu J, Liu P Tags: Am J Physiol Renal Physiol Source Type: research

Functional interaction of Junctophilin 2 with small ‐ conductance Ca2+‐activated potassium channel subtype 2(SK2) in mouse cardiac myocytes
ConclusionThe present data provide evidence that the functional interaction between JP2 and SK2 channels is present in the native mouse heart tissue. Junctophilin 2, as junctional membrane complex (JMC) protein, is an important regulator of the cardiac SK channels.
Source: Acta Physiologica - December 7, 2017 Category: Physiology Authors: H. K. Fan, T. X. Luo, W. D. Zhao, Y. H. Mu, Y. Yang, W. J. Guo, H. Y. Tu, Q. Zhang Tags: ORIGINAL ARTICLE Source Type: research

Functional interaction of Junctophilin 2 with small conductance Ca2+ ‐activated potassium channel subtype 2(SK2) in mouse cardiac myocytes
ConclusionsThe present data provide evidence that the functional interaction between JP2 and SK2 channels is present in the native mouse heart tissue. Junctophilin 2, as junctional membrane complex (JMC)protein, is an important regulator of the cardiac SK channels.This article is protected by copyright. All rights reserved.
Source: Acta Physiologica - October 21, 2017 Category: Physiology Authors: Hong K. Fan, Tian X. Luo, Wei D. Zhao, Yong H. Mu, Yang Yang, Wen J. Guo, Hui Y. Tu, Qian Zhang Tags: Regular Paper Source Type: research