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Proteomics evaluation of MDA-MB-231 breast cancer cells in response to RNAi-induced silencing of hPTTG
This study aims to evaluate cell proliferation and the downstream expression pattern of hPTTG1 gene at the mRNA and protein levels after specific down-regulation of hPTTG1 by siRNA.Main methodsThe human breast cancer MDA-MB-231 cell line was transfected with siRNA against hPTTG1. The mRNA and protein expression levels were examined by Real-time PCR and Western blot, respectively. The cell proliferation was assayed by MTS. To investigate the pattern of protein expression, total cellular protein was analyzed by 2D gel electrophoresis and mass spectroscopy. Subsequently, the possible biological consequences were determined by...
Source: Life Sciences - September 13, 2019 Category: Biology Source Type: research

Solid Phase Synthesis and Self ‐Assembly of Higher‐Order siRNAs and their Bioconjugates
This article is protected by copyright. All rights reserved.
Source: Chemical Biology and Drug Design - November 27, 2018 Category: Biology Authors: Christopher N. Cultrara, Sunil Shah, Stephen D. Kozuch, Mayurbhai R. Patel, David Sabatino Tags: Special Issue Article Source Type: research

Amphiphilic small peptides for delivery of plasmid DNAs and siRNAs
This article is protected by copyright. All rights reserved. As novel nucleic acid carriers, linear and branched amphiphilic peptides were prepared. Each peptide was compared in terms of SNALP size, stability, and delivery activities for plasmid DNA (pDNA) and siRNA. Among the five peptides, a linear peptide H‐RCL was an effective delivery carrier for both siRNA and pDNA. Introduction of a benzoyl group to H‐RCL significantly enhanced siRNA delivery, but greatly reduced pDNA delivery. These demonstrated that a small structural modification of the peptides can modulate their delivery properties.
Source: Chemical Biology and Drug Design - October 20, 2017 Category: Biology Authors: Eun ‐Kyoung Bang, Hanna Cho, Sean S‐H Jeon, Na Ly Tran, Dong‐Kwon Lim, Wooyoung Hur, Taebo Sim Tags: Research Article Source Type: research