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ERK/PKM2 Is Mediated in the Warburg Effect and Cell Proliferation in Arsenic-Induced Human L-02 Hepatocytes
This study aimed to investigate the potential role of pyruvate kinase M2 (PKM2) and extracellular regulated protein kinase (ERK) in arsenic-induced cell proliferation. L-02 cells were treated with 0.2 and 0.4 μmol/L As3+, glycolysis inhibitor (2-deoxy-D-glucose,2-DG), ERK inhibitor [1,4-diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)-butadiene, U0126] or transfected with PKM2 plasmid. Cell viability, proliferation, lactate acid production, and glucose intake capacity were determined by CCK-8 assay, EdU assay, lactic acid kit and 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2-NBDG) uptake kit, respectivel...
Source: Biological Trace Element Research - May 26, 2023 Category: Biology Authors: Fanshuo Yin Xin Zhang Zaihong Zhang Meichen Zhang Yunyi Yin Yanmei Yang Yanhui Gao Source Type: research

ZnT8 Exerts Anti-apoptosis of Kidney Tubular Epithelial Cell in Diabetic Kidney Disease Through TNFAIP3-NF- κB Signal Pathways
Biol Trace Elem Res. 2022 Jul 23. doi: 10.1007/s12011-022-03361-w. Online ahead of print.ABSTRACTApoptosis of kidney tubular epithelial cells contributes to diabetic kidney disease (DKD) pathophysiology, but the mechanisms are not fully understood. Zinc transporter protein member 8 (ZnT8, SLC30A8) is a susceptive gene in diabetes. Here, we aim to investigate whether ZnT8 has effects on pathophysiology of DKD. The animal groups include control, ZnT8KO mice, STZ-induced, and ZnT8-KO-STZ. STZ-induced DKD was performed in male C57BL/6 J mice and in ZnT8-KO mice. High glucose (HG)-induced apoptosis in a normal rat kidney tubula...
Source: Biological Trace Element Research - July 23, 2022 Category: Biology Authors: Yinmao Chi Xiuli Zhang Dan Liang Yue Wang Xiaoyi Cai Jiqiu Dong Lingzhi Li Zhihong Chi Source Type: research

HIF-1 α Mediates Arsenic-Induced Metabolic Reprogramming in Lung Bronchial Epithelial Cells
Biol Trace Elem Res. 2022 Jun 17. doi: 10.1007/s12011-022-03315-2. Online ahead of print.ABSTRACTArsenic is a common environmental pollutant that can cause damage to multiple systems and organs in the body. The lungs are particularly sensitive to arsenic exposure, and respiratory disease is thought to be the leading cause of death from arsenic poisoning. Our previous study found that human bronchial epithelial (HBE) cells treated with NaAsO2 exhibited mitochondrial dysfunction accompanied by elevated HIF-1α; however, the molecular mechanism was unclear. The aim of the current study was to confirm the role of HIF-1α in ar...
Source: Biological Trace Element Research - June 17, 2022 Category: Biology Authors: Wenjuan Wang Aihua Zhang Source Type: research

Vitamin C Attenuates Sodium Fluoride-Induced Mitochondrial Oxidative Stress and Apoptosis via Sirt1-SOD2 Pathway in F9 Cells.
Abstract Increasing evidence has suggested an important role played by reactive oxygen species (ROS) in the pathogenesis of fluorosis. Accumulating evidence demonstrates that vitamin C administration ameliorate sodium fluoride (NaF)-induced oxidative stress. However, the potentially beneficial effects of vitamin C against NaF-induced cytotoxicity and the underlying molecular mechanisms of this protection are not fully understood. Here, we found that NaF stimulated cytotoxicity, increased mitochondrial reactive oxygen species (mROS) production, and induced apoptosis in F9 embryonic carcinoma cells. Consistent with ...
Source: Biological Trace Element Research - December 18, 2018 Category: Biology Authors: Peng W, Xu S, Zhang J, Zhang Y Tags: Biol Trace Elem Res Source Type: research

Gene Silencing of Selenoprotein K Induces Inflammatory Response and Activates Heat Shock Proteins Expression in Chicken Myoblasts.
Abstract In the present study, specific small interfering RNA (siRNA) for selenoprotein K (Selk) gene was designed and transfected into chicken myoblasts. Then, the expressions of inflammatory factors (including induced nitric oxide synthase [iNOS], nuclear factor-kappa B [NF-κB], heme-oxygenase-1 [HO-1], cyclooxygenase-2 [COX-2], and prostaglandin E synthase [PTGEs]), inflammation-related cytokines (including interleukin [IL]-1β, IL-6, IL-7, IL-8, IL-17, and interferon [IFN]-γ), and heat shock proteins (HSPs) (including HSP27, HSP40, HSP60, HSP70, and HSP90) were examined at 24 and 72 h after transfection. Th...
Source: Biological Trace Element Research - March 8, 2017 Category: Biology Authors: Fan R, Yao H, Cao C, Zhao X, Khalid A, Zhao J, Zhang Z, Xu S Tags: Biol Trace Elem Res Source Type: research

Micromolar Levels of Sodium Fluoride Promote Osteoblast Differentiation Through Runx2 Signaling.
In this study, we characterized the effects of micromolar levels of NaF on the proliferation and osteogenic differentiation of MC3T3-E1 osteoblastic cells. NaF significantly enhanced the proliferation, alkaline phosphatase (ALP) activity, and mineralization of MC3T3-E1 cells. Quantitative real-time PCR analysis revealed that the expression of mRNAs encoding runt-related transcription factor 2 (Runx2), Osterix, Osteopontin and Osteocalcin was up-regulated in NaF-treated MC3T3-E1 cells compared with untreated controls. Western blot analysis demonstrated that Runx2 and Osterix were inhibited by Runx2 siRNA but were re-activat...
Source: Biological Trace Element Research - January 12, 2017 Category: Biology Authors: Lee M, Arikawa K, Nagahama F Tags: Biol Trace Elem Res Source Type: research

HNF-4alpha Negatively Regulates Hepcidin Expression Through BMPR1A in HepG2 Cells.
In conclusion, the present study suggests that HNF-4α has a suppressive effect on hepcidin expression by inactivating the BMP pathway, specifically via BMPR1A, in HepG2 cells. PMID: 27660075 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - September 22, 2016 Category: Biology Authors: Shi W, Wang H, Zheng X, Jiang X, Xu Z, Shen H, Li M Tags: Biol Trace Elem Res Source Type: research

Silencing of Human CutC Gene (hCutC) Induces Apoptosis in HepG2 Cells.
Abstract Copper is an essential microelement required for maintaining normal cell physiology. Copper transporter CutC is one of the six members of Cut family proteins, involved in prokaryotic copper homeostasis. Human homolog of CutC (hCutC) is an intracellular copper-binding protein with unknown physiological function. In the present study using HepG2 cells, we report the effects of hCutC knockdown on copper sensitivity and morphology of cells that ultimately leads to apoptosis. We silenced hCutC using specific small interfering RNA (siRNA), and its downregulation was confirmed by quantitative real-time PCR. Thou...
Source: Biological Trace Element Research - December 10, 2015 Category: Biology Authors: Kunjunni R, Sathianathan S, Behari M, Chattopadhyay P, Subbiah V Tags: Biol Trace Elem Res Source Type: research

Analysis of the Role of Insulin Signaling in Bone Turnover Induced by Fluoride.
In conclusion, insulin played the important role in bone lesion induced by excessive amount of fluoride through mediating InR receptor signaling, and IGF1 signaling probably exerted action on bone turnover caused by overdose of fluoride. PMID: 26521058 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - October 31, 2015 Category: Biology Authors: Liu Q, Liu H, Yu X, Wang Y, Yang C, Xu H Tags: Biol Trace Elem Res Source Type: research

Effect of siRNA PERK on Fluoride-Induced Osteoblastic Differentiation in OS732 Cells.
This study proved that the mechanism underlying fluoride induced osteoblastic and osteoclastic differentiation possible was due to activation of ALP and RANKL mediated by PERK in OS732 cells. PMID: 24838929 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - May 18, 2014 Category: Biology Authors: Lü P, Li X, Ruan L, Xu H, Liu Q Tags: Biol Trace Elem Res Source Type: research

Role of Unfolded Protein Response in Affecting Osteoblast Differentiation Induced by Fluoride.
In conclusion, fluoride had dual effect on osteogenic action. The UPR possibly involved in the mechanism of osteoblasts differentiation induced by fluoride. PMID: 24522478 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - February 13, 2014 Category: Biology Authors: Li XN, Lv P, Sun Z, Li GS, Xu H Tags: Biol Trace Elem Res Source Type: research

Effects of Zinc Transporter on Differentiation of Bone Marrow Mesenchymal Stem Cells to Osteoblasts.
Abstract The differentiation of bone marrow mesenchymal stem cells (MSCs) into osteoblasts is a crucial step during bone formation. However, the exact mechanisms regulating the early stages of osteogenic differentiation remain unknown. In the present study, we found that ZnT7, a member of the zinc transporter family SLC30A(ZnTs), was downregulated during dexamethasone-induced differentiation of rat MSCs into osteoblasts. Dexamethasone treatment resulted in significantly lower levels of ZnT7 compared with cocultured cells without dexamethasone. Differentiation was evaluated by measuring alkaline phosphatase (ALP) a...
Source: Biological Trace Element Research - June 18, 2013 Category: Biology Authors: Liu Y, Yan F, Yang WL, Lu XF, Wang WB Tags: Biol Trace Elem Res Source Type: research