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Thioredoxin reductase-1 levels are associated with NRF2 pathway activation and tumor recurrence in non-small cell lung cancer
Free Radic Biol Med. 2021 Oct 19;177:58-71. doi: 10.1016/j.freeradbiomed.2021.10.020. Online ahead of print.ABSTRACTActivating mutations in the KEAP1/NRF2 pathway characterize a subset of non-small cell lung cancer (NSCLC) associated with chemoresistance and poor prognosis. We herein evaluated the relationship between 64 oxidative stress-related genes and overall survival data from 35 lung cancer datasets. Thioredoxin reductase-1 (TXNRD1) stood out as the most significant predictor of poor outcome. In a cohort of NSCLC patients, high TXNRD1 protein levels correlated with shorter disease-free survival and distal metastasis-...
Source: Free Radical Biology and Medicine - October 21, 2021 Category: Biology Authors: Marina Delgobo Ros ângela Mayer Gonçalves Marco Ant ônio Delazeri Marcelo Falchetti Alessandro Zandon á Raquel Nascimento das Neves Karoline Almeida Adriane Cristina Fagundes Daniel Pens Gelain Jo ão Isidro Fracasso Guilherme Baroni de Mac êdo Leona Source Type: research

NALP3 orchestrates cellular bioenergetics to facilitate non-small cell lung cancer cell growth
This study was to unravel the mechanism of NALP3 on modulating NSCLC cancer cell growth.MethodsIHC and immuno-blot were performed to analyze expression of NALP3 and indicated molecules. CCK-8 and xenograft nude mice assay were used to evaluate cell growth in vitro and in vivo. Bioenergetics assay was performed to measure OXPHOS and aerobic glycolysis. siRNA and shRNA were constructed to knockdown endogenous NALP3 and DNMT1. Co-immunoprecipitation was applied to confirm the interaction between NALP3 and DMAP1. BioProfile FLEX analyzer and Lactate Reagent Kit were used to measure relative level glucose uptake and lactate pro...
Source: Life Sciences - December 14, 2019 Category: Biology Source Type: research

Cisplatin resistance involves a metabolic reprogramming through ROS and PGC-1 α in NSCLC which can be overcome by OXPHOS inhibition.
CONCLUSION: These results describe a new cisplatin resistance mechanism in NSCLC based on a metabolic reprogramming that is therapeutically exploitable through PGC-1α downregulation or OXPHOS inhibitors. PMID: 30880247 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - March 13, 2019 Category: Biology Authors: Cruz-Bermúdez A, Laza-Briviesca R, Vicente-Blanco RJ, García-Grande A, Coronado MJ, Laine-Menéndez S, Palacios-Zambrano S, Moreno-Villa MR, Ruiz-Valdepeñas AM, Lendinez C, Romero A, Franco F, Calvo V, Alfaro C, Acosta PM, Salas C, Garcia JM, Provencio Tags: Free Radic Biol Med Source Type: research

VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC.
In this study, VAOS exhibited effective inhibitory effects in NSCLC both in cultured cells and in a xenograft mouse model. VAOS was further identified to induce NSCLC cell apoptosis through activating protein kinase B (AKT) to elevate intracellular reactive oxygen species (ROS) levels by increasing in oxygen consumption and impairing the ROS-scavenging system. Neither silencing of PTP1B by siRNA nor transient overexpression of PTP1B had an effect on the AKT phosphorylation triggered by VAOS, indicating that PTP1B inhibition was not involved in VAOS-induced apoptosis. Through phosphorus colorimetric assay, we demonstrated t...
Source: Free Radical Biology and Medicine - September 14, 2018 Category: Biology Authors: Zhou L, Yi Y, Yuan Q, Zhang J, Li Y, Wang P, Xu M, Xie S Tags: Free Radic Biol Med Source Type: research

In vitro study of FUZ as a novel potential therapeutic target in non-small-cell lung cancer
Publication date: Available online 6 February 2018 Source:Life Sciences Author(s): Minwei He, Kangqi Li, Chuanfei Yu, Bingfeng Lv, Ning Zhao, Jinhai Deng, Lulu Cao, He Huang, Ang Yin, Taiping Shi, Lu Wang FUZ is regarded as a planar cell polarity effector that controls multiple cellular processes during vertebrate development. However, the role of FUZ in tumor biology remains poorly studied. Our purpose of this study is to discover the physiological effects and mechanism of FUZ in non-small-cell lung cancer (NSCLC) in vitro. With the help of bioinformatics analysis, we noticed that the expression level of FUZ negatively c...
Source: Life Sciences - February 7, 2018 Category: Biology Source Type: research

7-O-geranylquercetin-induced autophagy contributes to apoptosis via ROS generation in human non-small cell lung cancer cells
Publication date: Available online 8 May 2017 Source:Life Sciences Author(s): En-Xia Wang, Bo-Yang Zou, Lei Shi, Lin-Ying Du, Yan-Yan Zhu, Ya-Meng Jiang, Xiao-Dong Ma, Xiao-Hui Kang, Chang-Yuan Wang, Yu-Hong Zhen, Li-Dan Sun Aims To investigate the antitumor effects of 7-O-geranylquercetin (GQ), a novel O-alkylated derivative of quercetin, against non-small cell lung cancer (NSCLC) cell lines A549 and NCI-H1975 and the corresponding mechanisms. Main methods Cell viability was assessed using MTT assay. The expression of proteins involved in apoptosis and autophagy was measured using western blotting. Besides, apoptosis was...
Source: Life Sciences - May 9, 2017 Category: Biology Source Type: research

Oxaliplatin activates the KEAP1/NRF2 antioxidant system conferring protection against the cytotoxicity of anticancer drugs.
Abstract Oxaliplatin is an important drug in the treatment of advanced metastatic colorectal cancer. NF-E2 P45-related factor 2 (NRF2) is a key transcription factor that controls genes encoding cytoprotective and detoxifying enzymes through antioxidant response elements (AREs) in their regulatory regions. Here, we report that oxaliplatin is an activator of the NRF2 signaling pathway, with up-regulation of ARE-driven genes and glutathione elevation. An injection of oxaliplatin into mice enhanced the expression of glutathione transferases and antioxidant enzymes in the small and large intestines of wild-type (WT) mi...
Source: Free Radical Biology and Medicine - February 17, 2014 Category: Biology Authors: Wang XJ, Li Y, Luo L, Wang H, Chi Z, Xin A, Li X, Wu J, Tang X Tags: Free Radic Biol Med Source Type: research