• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Molecules, Vol. 27, Pages 4377: Tetrazine-Induced Bioorthogonal Activation of Vitamin E-Modified siRNA for Gene Silencing
Xinjing Tang The temporal activation of siRNA provides a valuable strategy for the regulation of siRNA activity and conditional gene silencing. The bioorthogonal bond-cleavage reaction of benzonorbonadiene and tetrazine is a promising trigger in siRNA temporal activation. Here, we developed a new method for the bio-orthogonal chemical activation of siRNA based on the tetrazine-induced bond-cleavage reaction. Small-molecule activatable caged siRNAs were developed with the 5′-vitamin E-benzonobonadiene-modified antisense strand targeting the green fluorescent protein (GFP) gene and the mitotic kinesin-5 ...
Source: Molecules - July 8, 2022 Category: Chemistry Authors: Xueli Zhang Amu Gubu Jianfei Xu Ning Yan Wenbo Su Di Feng Qian Wang Xinjing Tang Tags: Article Source Type: research

Advances in nanomicelles for sustained drug delivery
Publication date: 25 November 2017 Source:Journal of Industrial and Engineering Chemistry, Volume 55 Author(s): Najmeh Amirmahani, Nosrat O. Mahmoodi, Mohsen Mohammadi Galangash, Atefeh Ghavidast The efficient direct access of therapeutic molecules to the target tissues is still a major obstacle in developing useful therapeutic interventions. Over the past decades, polymeric micelles are interesting nanocarries for drug delivery systems due to small size (10–100nm), low toxicity, capacity to solubilize lipophilic drugs in core and high drug loading. Here, the design of various nanomicelles such as amphiphilic dendrimer,...
Source: Journal of Industrial and Engineering Chemistry - August 5, 2017 Category: Chemistry Source Type: research

Autocrine and Paracrine Interactions between Multiple Myeloma Cells and Bone Marrow Stromal Cells by Growth Arrest-specific Gene 6 Cross-talk with Interleukin-6 Signal Transduction
The pathogenesis of multiple myeloma (MM) has not yet been fully elucidated. Our microarray analysis and immunohistochemistry revealed significant up-regulation of growth arrest-specific gene 6 (Gas6), a vitamin K-dependent protein with a structural homology with protein S, in bone marrow (BM) cells of MM patients. ELISA showed that the serum levels of soluble Gas6 were significantly increased in the MM patients when compared with healthy controls. Gas6 was overexpressed in the human CD138-positive MM cell line RPMI-8226. Exogenous Gas6 suppressed apoptosis induced by serum deprivation and enhanced cell proliferation of th...
Source: Journal of Biological Chemistry - March 9, 2017 Category: Chemistry Authors: Miki Furukawa, Hiroshi Ohkawara, Kazuei Ogawa, Kazuhiko Ikeda, Koki Ueda, Akiko Shichishima-Nakamura, Emi Ito, Jun-ichi Imai, Yuka Yanagisawa, Reiko Honma, Shinya Watanabe, Satoshi Waguri, Takayuki Ikezoe, Yasuchika Takeishi Tags: Cell Biology Source Type: research

ATRA Stimulates DRA Expression Signal Transduction
In conclusion, ATRA may act as an antidiarrheal agent by increasing DRA expression via the RAR-β/HNF-1β-dependent pathway.
Source: Journal of Biological Chemistry - June 11, 2015 Category: Chemistry Authors: Priyamvada, S., Anbazhagan, A. N., Gujral, T., Borthakur, A., Saksena, S., Gill, R. K., Alrefai, W. A., Dudeja, P. K. Tags: Cell Biology Source Type: research

Cystathionine-{gamma}-lyase Activity in Type 1 Diabetes Molecular Bases of Disease
This study investigated the activities of cystathionine-γ-lyase (CSE, the enzyme that catalyzes H2S formation) in livers of type 1 diabetic (T1D) animals and in peripheral blood mononuclear cells (PBMC) isolated from T1D patients. T1D is associated with both hyperketonemia (acetoacetate and β-hydroxybutyrate) and hyperglycemia. This study also examined the role of hyperglycemia and hyperketonemia per se in decreased CSE activity using U937 monocytes and PBMC isolated from healthy subjects. Livers from streptozotocin-treated T1D rats demonstrated a significantly higher reactive oxygen species production, lower CSE protein...
Source: Journal of Biological Chemistry - April 25, 2014 Category: Chemistry Authors: Manna, P., Gungor, N., McVie, R., Jain, S. K. Tags: Metabolism Source Type: research

REPORT: Ascorbate Enhances the Generation of 5-hmC DNA and Chromosomes
Ascorbate (vitamin C) is best known for its role in scurvy, in which the hydroxylation of collagen catalyzed by dioxygenases is incomplete due to ascorbate deficiency. Here, we report a novel function of ascorbate in the hydroxylation of 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA catalyzed by Tet (ten-eleven translocation) methylcytosine dioxygenase. The content of 5-hmC is extremely low in mouse embryonic fibroblasts cultured in ascorbate-free medium. Additions of ascorbate dose- and time-dependently enhance the generation of 5-hmC, without any effects on the expression of Tet genes. Treatment with ...
Source: Journal of Biological Chemistry - May 10, 2013 Category: Chemistry Authors: Minor, E. A., Court, B. L., Young, J. I., Wang, G. Tags: Reports Source Type: research