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Regulation of BMP2‐induced intracellular calcium increases in osteoblasts
In this study, intracellular Ca2+ concentration ([Ca2+]i) upon BMP2 application, focal adhesion kinase (FAK) and Src activities were measured in the MC3T3‐E1 osteoblast cell line using fluorescence resonance energy transfer‐based biosensors. Increase in [Ca2+]i, FAK, and Src activities were observed during BMP2 stimulation. The removal of extracellular calcium, the application of membrane channel inhibitors streptomycin or nifedipine, the FAK inhibitor PF‐573228 (PF228), and the alkaline phosphatase (ALP) siRNA all blocked the BMP2‐stimulated [Ca2+]i increase, while the Src inhibitor PP1 did not. In contrast, a gen...
Source: Journal of Orthopaedic Research - March 4, 2016 Category: Orthopaedics Authors: Wenfeng Xu, Bo Liu, Xue Liu, Martin Y. M. Chiang, Bo Li, Zichen Xu, Xiaoling Liao Tags: Research Article Source Type: research

Heterozygous PTCH1 Mutations Impact the Bone Metabolism in Patients with Nevoid Basal Cell Carcinoma Syndrome Likely by Regulating SPARC Expression
This study sought to investigate the mechanism underlying heterozygous PTCH1 mutation‐mediated abnormal bone metabolism in patients with NBCCS. Stromal cells were isolated from the fibrous capsules of patients with NBCCS‐associated or non‐syndromic keratocystic odontogenic tumors and non‐syndromic tumor stromal cells without PTCH1 mutations served as controls. Germline PTCH1 heterozygous mutations were confirmed in all NBCCS samples and differential protein expression was identified using tandem mass tag‐labeled proteomics analysis. Our findings revealed that osteonectin/SPARC expression was significantly downreg...
Source: Journal of Bone and Mineral Research - February 17, 2016 Category: Orthopaedics Authors: Yingying Hong, Jianyun Zhang, Heyu Zhang, Xuefen Li, Jiafei Qu, Jiemei Zhai, Lei Zhang, Feng Chen, Tiejun Li Tags: Original Article Source Type: research

The regulation of BMP2‐induced intracellular calcium increases in osteoblasts
This article is protected by copyright. All rights reserved
Source: Journal of Orthopaedic Research - February 1, 2016 Category: Orthopaedics Authors: Wenfeng Xu, Bo Liu, Xue Liu, Martin Chiang, Bo Li, Zichen Xu, Xiaoling Liao Tags: Research Article Source Type: research

Expression of Caveolin-1 in Periodontal Tissue and Its Role in Osteoblastic and Cementoblastic Differentiation In Vitro
This study demonstrates, for the first time, that Cav-1 is expressed in developing periodontal tissue and in vitro in periodontal-related cells. Cav-1 inhibition positively regulates osteoblastic differentiation in hPDLCs, cementoblasts, and osteoblasts via BMP, AMPK, MAPK, and NF-κB pathway. Thus, Cav-1 inhibition may be a novel molecular target for therapeutic approaches in periodontitis or osteolytic disease.
Source: Calcified Tissue International - December 19, 2015 Category: Orthopaedics Source Type: research

Role of the parathyroid hormone type 1 receptor (PTH1R) as a mechanosensor in osteocyte survival
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - December 1, 2014 Category: Orthopaedics Authors: Marta Maycas, Juan A. Ardura, Luis Fernández de Castro, Beatriz Bravo, Arancha R. Gortázar, Pedro Esbrit Tags: Original Article Source Type: research

Effects of hyperbaric oxygen on the osteogenic differentiation of mesenchymal stem cells
Conclusion: HBO treatment increased osteogenic differentiation of MSCs via regulating Wnt processing, secretion, and signaling.
Source: BMC Musculoskeletal Disorders - February 25, 2014 Category: Orthopaedics Authors: Song-Shu LinSteve UengChi-Chien NiuLi-Jen YuanChuen-Yung YangWen-Jer ChenMel LeeJan-Kan Chen Source Type: research