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Specialty: Opthalmology
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Total 104 results found since Jan 2013.
Purinergic 2X 4 (P2X4), but not P2X7, receptors increase cytosolic Ca < sup > 2+ < /sup > and stimulate mucin secretion in rat conjunctival goblet cells to maintain ocular surface health
Exp Eye Res. 2023 Aug 12:109614. doi: 10.1016/j.exer.2023.109614. Online ahead of print.ABSTRACTIonotropic purinergic receptors (P2XRs) are activated by ATP and ATP analogs. ATP can be released through ATP-permeable channels such as the pannexin hemichannels. Upon activation, the P2XRs become permeable to Ca2+, a potent stimulator of mucin secretion in conjunctival goblet cells (CGCs). The purpose of this study was to investigate the presence and function of P2XRs in CGCs. We also examined the presence of pannexin hemichannels. Rat first passage CGCs were stained with the goblet cell marker anti-cytokeratin 7 antibody and ...
Source: Experimental Eye Research - August 14, 2023 Category: Opthalmology Authors: Haakon K Fj ærvoll Ketil A Fj ærvoll Menglu Yang Jeffrey Bair Tor P Utheim Darlene A Dartt Source Type: research
Heparanase-mediated histone 3 acetylation regulates VEGF gene transcription in the hyperglycemia and hypoxia human retinal endothelial cells
Exp Eye Res. 2023 Jun 3:109519. doi: 10.1016/j.exer.2023.109519. Online ahead of print.ABSTRACTHeparanase (HPA) is believed that might mediate histone 3 lysine 9 acetylation (H3K9ac) to regulate vascular endothelial growth factor (VEGF) gene expressions in the hyperglycemia and hypoxia human retinal endothelial cells (HRECs). Cultured human retinal endothelial cells (HRECs) in hyperglycemia, hypoxia, siRNA, and normal medium, respectively. Distributions of H3K9ac and HPA in HRECs were analyzed by immunofluorescence. Western blot and real-time PCR were respectively used to evaluate the expression of HPA, H3K9ac, and VEGF. T...
Source: Experimental Eye Research - June 5, 2023 Category: Opthalmology Authors: Xuan Leng Jingwei Wang Xin Song Jie Hu Lin Lu Source Type: research
Inhibition of AIF-1 alleviates laser-induced macular neovascularization by inhibiting endothelial cell proliferation via restrained p44/42 MAPK signaling pathway
This study aimed to understand how AIF-1 effects the proliferation of CECs and the subsequent progression of MNV. To study this, a mouse MNV model was established through laser injury, and the AIF-1 expression levels were then measured using western blot and immunohistochemistry. AIF-1 siRNA was intravitreally injected to silence AIF-1 gene expression. Western blot and choroidal flat mount were performed to measure the progression of MNV and proliferation of the CECs. These results showed that the protein expression of AIF-1 was significantly elevated in the laser-induced mouse MNV model, and the expression trend was consi...
Source: Experimental Eye Research - April 20, 2023 Category: Opthalmology Authors: Qinghua Li Liting Hu Guibo Liu Xiaoni Yin Ying Li Xiangyang Wei Ning Duan Xiaoran Zhao Qingyun Gong Zhaodong Du Source Type: research
Complement 3a Mediates CCN2/CTGF in Human Retinal Pigment Epithelial Cells
CONCLUSIONS: C3 was elevated in RPE under environmental stimulus and long-term exposure to specified concentration of C3a increased CCN2/CTGF expression in RPE, which could be partially reversed by C3 siRNA.PMID:36276919 | PMC:PMC9581697 | DOI:10.1155/2022/3259453
Source: Journal of Ophthalmology - October 24, 2022 Category: Opthalmology Authors: Kang Xiao Zhiyan Xu Zhengyu Chen Qin Long Youxin Chen Source Type: research
siRNA high throughput screening identifies regulators of chloropicrin and hydrogen fluoride injury in human corneal epithelial cell models
Exp Eye Res. 2022 Jul 9:109169. doi: 10.1016/j.exer.2022.109169. Online ahead of print.ABSTRACTCorneal injuries induced by various toxicants result in similar clinical presentations such as corneal opacity and neovascularization. Many studies suggest that several weeks post-exposure a convergence of the molecular mechanisms drives these progressive pathologies. However, chemical agents vary in toxicological properties, and early molecular responses are anticipated to be somewhat dissimilar for different toxicants. We chose 3120 targets from the Dharmacon Human Druggable genome to screen for chloropicrin (CP) and hydrogen f...
Source: Experimental Eye Research - July 12, 2022 Category: Opthalmology Authors: Albert L Ruff Robert D Causey Jeffrey A Koenig Cristina V LaGrasta Amber M Gomez Bailey T Chalmers John G Lehman Source Type: research
Suppression of EZH2 inhibits TGF- β1-induced EMT in human retinal pigment epithelial cells
This study aimed to investigate the role of enhancer of zeste homolog 2 (EZH2) in EMT of human primary RPE cells and the underlying mechanisms of the anti-fibrotic effect of EZH2 suppression. Primary cultures of human RPE cells were treated with TGF-β1 for EMT induction. EZH2 was silenced by siRNA to assess the expression levels of epithelial and fibrotic markers using qRT-PCR, Western blot, and immunofluorescence staining assay. Furthermore, the cellular migration, proliferation and barrier function of RPE cells were evaluated. RNA-sequencing analyses were performed to investigate the underlying mechanisms of EZH2 inhibi...
Source: Experimental Eye Research - July 5, 2022 Category: Opthalmology Authors: Yu Peng Kai Liao Feng Tan Yuqin Liang Xihao Sun Zekai Cui Bo Ye Zhongping Chen Shibo Tang Jiansu Chen Source Type: research
