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The long and short: Non-coding RNAs in the mammalian inner ear
Hear Res. 2022 Dec 16;428:108666. doi: 10.1016/j.heares.2022.108666. Online ahead of print.ABSTRACTNon-coding RNAs (ncRNAs) play a critical role in the entire body, and their mis-regulation is often associated with disease. In parallel with the advances in high-throughput sequencing technologies, there is a great deal of focus on this broad class of RNAs. Although these molecules are not translated into proteins, they are now well established as significant regulatory components in many biological pathways and pathological conditions. ncRNAs can be roughly divided into two main sub-groups based on the length of the transcr...
Source: Hearing Research - December 25, 2022 Category: Audiology Authors: Tal Koffler-Brill Yael Noy Karen B Avraham Source Type: research

Id1/NR2B Receptor Pathway Regulates Rat Cochlear Sensory Epithelial Cell Survival after Radiation
Survival of cochlear sensory epithelial cells may be regulated by inhibitor of differentiation-1 (Id1) and the N-methyl-D-aspartic acid (NMDA) receptor. However, it is unclear whether Id1 and the NMDA receptor are involved in the radiation-mediated survival of rat cochlear sensory epithelial cells. Here, we show that the percentage of apoptotic cells increased, the percentage of cells in the S phase decreased, Id1 mRNA and protein expression decreased and the NMDA receptor subtype 2B (NR2B) mRNA and protein level increased in OC1 cells after radiation. Cells infected with the Id1 gene exhibited higher Id1 mRNA and protein ...
Source: Audiology and Neurotology - October 9, 2018 Category: Audiology Source Type: research

miR-204 suppresses cochlear spiral ganglion neuron survival in vitro by targeting TMPRSS3.
Abstract Sensorineural hearing loss (SNHL) is the most common cause of hearing impairment. One of the essential steps to prevent progressive hearing loss is to protect spiral ganglion neurons (SGNs) from ongoing degeneration. MicroRNAs and TMPRSS3 (transmembrane protease, serine 3) have been reported to be involved in development of SGNs and genesis of SNHL. The aim of this study was to investigate the role of miR-204 and TMPRSS3 in SGNs. Effect of miR-204 on cell viability of SGNs was first examined using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Expression of TMPRSS3 in SGNs with...
Source: Hearing Research - June 9, 2014 Category: Audiology Authors: Li Y, Peng A, Ge S, Wang Q, Liu J Tags: Hear Res Source Type: research

Regeneration of mammalian cochlear and vestibular hair cells through Hes1/Hes5 modulation with siRNA.
Abstract The Notch pathway is a cell signaling pathway determining initial specification and subsequent cell fate in the inner ear. Previous studies have suggested that new hair cells (HCs) can be regenerated in the inner ear by manipulating the Notch pathway. In the present study, delivery of siRNA to Hes1 and Hes5 using a transfection reagent or siRNA to Hes1 encapsulated within poly(lactide-co-glycolide acid) (PLGA) nanoparticles increased HC numbers in non-toxin treated organotypic cultures of cochleae and maculae of postnatal day 3 mouse pups. An increase in HCs was also observed in cultured cochleae and macu...
Source: Hearing Research - July 9, 2013 Category: Audiology Authors: Du X, Li W, Gao X, West MB, Mark Saltzman W, Cheng CJ, Stewart C, Zheng J, Cheng W, Kopke RD Tags: Hear Res Source Type: research

Intra-tympanic delivery of short interfering RNA into the adult mouse cochlea.
Abstract Trans-tympanic injection into the middle ear has long been the standard for local delivery of compounds in experimental studies. Here we demonstrate the advantages of the novel method of intra-tympanic injection through the otic bone for the delivery of compounds or siRNA into the adult mouse cochlea. First, a fluorescently-conjugated scrambled siRNA probe was applied via intra-tympanic injection into the middle ear cavity and was detected in sensory hair cells and nerve fibers as early as 6 h after the injection. The fluorescent probe was also detected in other cells of the organ of Corti, the lateral w...
Source: Hearing Research - November 23, 2012 Category: Audiology Authors: Oishi N, Chen FQ, Zheng HW, Sha SH Tags: Hear Res Source Type: research