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SUMO1 depletion prevents lipid droplet accumulation and HCV replication
Abstract Infection by hepatitis C virus (HCV) is a major public-health problem. Chronic infection often leads to cirrhosis, steatosis, and hepatocellular carcinoma. The life cycle of HCV depends on the host cell machinery and involves intimate interaction between viral and host proteins. However, the role of host proteins in the life cycle of HCV remains poorly understood. Here, we identify the small ubiquitin-related modifier (SUMO1) as a key host factor required for HCV replication. We performed a series of cell biology and biochemistry experiments using the HCV JFH-1 (Japanese fulminate hepatitis 1) genotype 2a...
Source: Archives of Virology - October 8, 2015 Category: Virology Source Type: research

The role of Moloney leukemia virus 10 in hepatitis B virus expression in hepatoma cells.
In this study, Mov10 was demonstrated to affect HBV expression in HepG2 and HepG2.2.15 cell lines. The data showed that the over-expression of exogenous Mov10 resulted in an increase of the HBsAg and HBeAg levels in the culture supernatant and HBV mRNA level in transfected cells at a low dose and resulted in a decrease at a high dose, but HBV DNA in culture supernatant was not affected. The knockdown of endogenous Mov10 expression through siRNA treatment could suppress levels of HBsAg, HBeAg and HBV mRNA, but had no effect on HBV DNA. Above results indicate that an appropriate level of exogenous Mov10 is responsible for HB...
Source: Virus Research - December 19, 2014 Category: Virology Authors: Ma Y, Li D, Fu L, Fu B, Chen S, Xu W, Teng X, Song Z, Gu H Tags: Virus Res Source Type: research

BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells.
Abstract Hepatitis C virus (HCV) infection is a common cause of chronic hepatitis and is currently treated with alpha interferon (IFN-α)-based therapies. IFN-induced cell membrane protein BST2 (also known as CD317, HM1.24 or tetherin) has been reported to tether a broad range of lipid-enveloped viruses on cell surfaces. However, whether HCV is sensitive to BST2 remains controversial. Here we established a Huh7.5-BST2-TO cell line, in which BST2 expression is regulated by tetracycline. Our results showed that the effect of BST2 on inhibiting HCV production was dependent on its expression level. Highly expressed BS...
Source: Antiviral Research - February 16, 2013 Category: Virology Authors: Pan XB, Qu XW, Jiang D, Zhao XL, Han JC, Wei L Tags: Antiviral Res Source Type: research