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Gene therapy: Comprehensive overview and therapeutic applications
Life Sci. 2022 Feb 3:120375. doi: 10.1016/j.lfs.2022.120375. Online ahead of print.ABSTRACTGene therapy is the product of man's quest to eliminate diseases. Gene therapy has three facets namely, gene silencing using siRNA, shRNA and miRNA, gene replacement where the desired gene in the form of plasmids and viral vectors, are directly administered and finally gene editing based therapy where mutations are modified using specific nucleases such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regulatory interspaced short tandem repeats (CRISPR)/CRISPR-associated protein ...
Source: Cell Research - February 6, 2022 Category: Cytology Authors: Nilofer Sayed Prince Allawadhi Amit Khurana Vishakha Singh Umashanker Navik Sravan Kumar Pasumarthi Isha Khurana Anil Kumar Banothu Ralf Weiskirchen Kala Kumar Bharani Source Type: research

Ultrasound-targeted microbubble destruction of calcium channel subunit  α 1D siRNA inhibits breast cancer via G protein-coupled receptor 30.
In conclusion, E2 upregulated the expression of Cav1.3 for Ca2+ influx to promote the expression of p-ERK1/2 for cell proliferation. The study confirmed that the mechanism of E2 inducing the expression of Cav1.3 through a non-genomic pathway, and highlighted that UTMD of Cav1.3 siRNA is a powerful promising technology for breast cancer gene therapy. PMID: 27572936 [PubMed - as supplied by publisher]
Source: Oncology Reports - September 1, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

A new method of ultrasonic nonviral gene delivery to the adult myocardium
Abstract: Cardiac gene transfer is a powerful molecular tool to improve our understanding of the role of new proteins and mutants in cardiac pathophysiology. There is a need for a simple efficient myocardial gene delivery technique in order to study the physiological role of proteins in their native environment. Here we tested a new method of myocardial nonviral gene delivery, by using the combination of ultrasound energy (USE), liposomes and high pressure injections to the rat heart. Wistar rats were subjected to intra-myocardial injections of liposomes-DNA or siRNA mix. The heart was exposed after an inter-costal incisio...
Source: Journal of Molecular and Cellular Cardiology - August 6, 2012 Category: Cytology Authors: Youakim Saliba, Nathalie Mougenot, Adeline Jacquet, Fabrice Atassi, Stéphane Hatem, Nassim Farès, Anne-Marie Lompré Tags: Original Articles Source Type: research