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mTORC2 ‐AKT‐LAT1 signalling participates in methionine‐induced β‐CASEIN expression in mammary epithelial cells of dairy cows
This study investigated the role of the mammalian target of rapamycin complex 2 (mTORC2)-protein kinase B (AKT) signalling in methionine (Met)-induced L-type amino acid transporter 1 (LAT1) expression and milk protein production. Primary mammary epithelial cells (MECs) from mammary parenchymal tissues of three lactating cows and MAC-T bovine MECs were cultured with or without 0.6  mM Met. Rapamycin-insensitive companion of mTOR (RICTOR) siRNA, the mTORC1 inhibitor rapamycin and the AKT activator SC79 were used to evaluate the effects of mTORC2-AKT signalling on Met-induced LAT1 expression and function. Each experiment wa...
Source: Journal of Animal Physiology and Animal Nutrition - April 6, 2023 Category: Zoology Authors: Xiaoyu Duan, Chuanping Liu, Xiaoqing Gong, Yang Yang, Hongtao Jiao, Ye Lin, Xiaoming Hou Tags: ORIGINAL ARTICLE Source Type: research

mTORC2 ‐AKT‐LAT1 Signalling Participates in Methionine‐induced β‐CASEIN Expression in Mammary Epithelial Cells of Dairy Cows
This study investigated the role of the mammalian target of rapamycin complex 2 (mTORC2)-protein kinase B (AKT) signalling in methionine (Met)-induced L-type amino acid transporter 1 (LAT1) expression and milk protein production. Primary mammary epithelial cells (MECs) from mammary parenchymal tissues of 3 lactating cows and MAC-T bovine MECs were cultured with or without 0.6 mM Met. Rapamycin-insensitive companion of mTOR (RICTOR) siRNA, the mTORC1 inhibitor rapamycin and the AKT activator SC79 were used to evaluate the effects of mTORC2-AKT signalling on Met-induced LAT1 expression and function. Each experiment was perfo...
Source: Journal of Animal Physiology and Animal Nutrition - March 25, 2023 Category: Zoology Authors: Xiaoyu Duan, Chuanping Liu, Xiaoqing Gong, Yang Yang, Hongtao Jiao, Ye Lin, Xiaoming Hou Tags: ORIGINAL ARTICLE Source Type: research