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Total 20 results found since Jan 2013.

Knock-down of aryl hydrocarbon receptor (AhR) affects the lncRNA-mediated response of porcine granulosa cells (AVG-16 cell line) to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
Anim Reprod Sci. 2023 Jun 7;255:107277. doi: 10.1016/j.anireprosci.2023.107277. Online ahead of print.ABSTRACTRecently, we found that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) - the most toxic dioxin - affected multiple cellular processes in AhR-knocked-down granulosa cells, including the expression of genes and the abundance of proteins. Such alterations may imply the involvement of noncoding RNAs in the remodeling of intracellular regulatory tracks. The aims of the current study were to examine the effects of TCDD on the expression of lncRNAs in AhR-knocked-down granulosa cells of pigs and to indicate potential target g...
Source: Animal Reproduction Science - June 14, 2023 Category: Zoology Authors: Sylwia Swigonska Anna Nynca Tomasz Molcan Monika Jablonska Renata E Ciereszko Source Type: research

mTORC2 ‐AKT‐LAT1 signalling participates in methionine‐induced β‐CASEIN expression in mammary epithelial cells of dairy cows
This study investigated the role of the mammalian target of rapamycin complex 2 (mTORC2)-protein kinase B (AKT) signalling in methionine (Met)-induced L-type amino acid transporter 1 (LAT1) expression and milk protein production. Primary mammary epithelial cells (MECs) from mammary parenchymal tissues of three lactating cows and MAC-T bovine MECs were cultured with or without 0.6  mM Met. Rapamycin-insensitive companion of mTOR (RICTOR) siRNA, the mTORC1 inhibitor rapamycin and the AKT activator SC79 were used to evaluate the effects of mTORC2-AKT signalling on Met-induced LAT1 expression and function. Each experiment wa...
Source: Journal of Animal Physiology and Animal Nutrition - April 6, 2023 Category: Zoology Authors: Xiaoyu Duan, Chuanping Liu, Xiaoqing Gong, Yang Yang, Hongtao Jiao, Ye Lin, Xiaoming Hou Tags: ORIGINAL ARTICLE Source Type: research

mTORC2 ‐AKT‐LAT1 Signalling Participates in Methionine‐induced β‐CASEIN Expression in Mammary Epithelial Cells of Dairy Cows
This study investigated the role of the mammalian target of rapamycin complex 2 (mTORC2)-protein kinase B (AKT) signalling in methionine (Met)-induced L-type amino acid transporter 1 (LAT1) expression and milk protein production. Primary mammary epithelial cells (MECs) from mammary parenchymal tissues of 3 lactating cows and MAC-T bovine MECs were cultured with or without 0.6 mM Met. Rapamycin-insensitive companion of mTOR (RICTOR) siRNA, the mTORC1 inhibitor rapamycin and the AKT activator SC79 were used to evaluate the effects of mTORC2-AKT signalling on Met-induced LAT1 expression and function. Each experiment was perfo...
Source: Journal of Animal Physiology and Animal Nutrition - March 25, 2023 Category: Zoology Authors: Xiaoyu Duan, Chuanping Liu, Xiaoqing Gong, Yang Yang, Hongtao Jiao, Ye Lin, Xiaoming Hou Tags: ORIGINAL ARTICLE Source Type: research

Suppression of MyoD induces spontaneous adipogenesis in skeletal muscle progenitor cell culture
AbstractThe degree of intramuscular adipose tissue accumulation is one of the factors affecting meat quality. Accumulation of adipocytes is also observed under the pathological condition of skeletal muscle such as muscular dystrophy and sarcopenia. The origin of adipocytes seen in skeletal muscle is mesenchymal progenitor cells that can give rise to both adipocytes and fibroblasts. In the present study, we demonstrated that siRNA-mediated suppression of MyoD expression in rat skeletal muscle progenitor cell culture, which comprises both myogenic satellite cells and mesenchymal progenitor cells, resulted in diminished myotu...
Source: Animal Science Journal - July 7, 2021 Category: Zoology Authors: Keitaro Yamanouchi, Katsuyuki Nakamura, Shiho Takeuchi, Tohru Hosoyama, Takashi Matsuwaki, Masugi Nishihara Tags: RESEARCH ARTICLE Source Type: research

Molecular characteristics and abundance of insulin-like androgenic gland hormone and effects of RNA interference in Eriocheir sinensis.
In this study, there was cloning of the full-length sequence of IAG from Eriocheir sinensis (Es-IAG). The full-length Es-IAG gene was 1392 base pairs long and encoded a protein of 151 amino acid residues. The precursor peptide included a signal peptide, and the protein was a protein that is secreted from the cell in which it is produced with no transmembrane domain. Amino acid sequence alignment indicated there was the greatest homology between E. sinensis and Chaceon quinquedens (47 %), followed by Callinectes sapidus (44 %). Results from analysis of the relative abundances of Es-IAG mRNA transcript at different developme...
Source: Animal Reproduction Science - March 30, 2020 Category: Zoology Authors: Fu C, Li F, Wang L, Wu F, Wang J, Fan X, Liu T Tags: Anim Reprod Sci Source Type: research

Effects of abundances of OCT-4 mRNA transcript on goat pre-implantation embryonic development.
Abstract Unlike in mice, the function of pluripotent markers in early embryonic development of domestic animals remains to be elucidated and this may account for the failure to establish embryonic stem cell lines for these species. To study the functions of the OCT-4 protein which has important actions in maintenance of pluripotent and self-renewal processes during early embryonic development, there was induced reduction in relative abundance of OCT-4 mRNA transcript during goat early embryonic development by using RNA interference techniques. The injection of OCT-4 siRNA into goat IVF presumptive zygotes resulted...
Source: Animal Reproduction Science - March 30, 2020 Category: Zoology Authors: Naddafpour A, Ghazvini Zadegan F, Hajian M, Hosseini SM, Jafarpour F, Rahimi M, Habibi R, Nasr Esfahani MH Tags: Anim Reprod Sci Source Type: research

Suppression of COX-2 mRNA abundance in in vitro cultured goat (Capra hircus) endometrial cells by RNA interference and effect on PGF2- α and PGE2 concentrations.
Suppression of COX-2 mRNA abundance in in vitro cultured goat (Capra hircus) endometrial cells by RNA interference and effect on PGF2-α and PGE2 concentrations. Anim Reprod Sci. 2019 Oct;209:106146 Authors: Suresh A, Reddy IJ, Mishra A, Mondal S Abstract Cyclooxygenase-2 (COX-2) has important functions in the synthesis and release of endometrial prostaglandin F2α (PGF2α). Excessive production of COX-2 leads to an increase in endometrial PGF2α synthesis and subsequently causes luteolysis and early embryonic mortality. The aim of this study was to investigate in goats the effects of COX-2 small inte...
Source: Animal Reproduction Science - September 14, 2019 Category: Zoology Authors: Suresh A, Reddy IJ, Mishra A, Mondal S Tags: Anim Reprod Sci Source Type: research

Mild heat stress induces transcription of the β‐casein gene via unfolded protein response‐activated XBP1 signaling in undifferentiated mammary epithelial cells
In this study, we investigated the relationship between an increase in β‐casein expression and the unfolded protein response (UPR) under mild heat stress. After reaching confluence, HC11 cells were incubated at 37°C (control) or 39°C (mild heat stress) without differentiation medium, and the expression levels of β‐casein and UPR‐related genes were assessed. It was revea led that, even with this mild heat treatment (39°C), β‐casein expression in HC11 cells increased at the transcriptional level without differentiation induction. The expression levels of X‐box binding protein 1 (XBP1) and activating transcrip...
Source: Animal Science Journal - June 13, 2019 Category: Zoology Authors: Moeko Mizusawa, Mst Mamuna Sharmin, Shinichi Yonekura Tags: ORIGINAL ARTICLE Source Type: research

MiR ‐222 inhibits apoptosis in porcine follicular granulosa cells by targeting the THBS1 gene
AbstractApoptosis of granulosa cells affects follicular atresia and reproduction and is regulated by miRNAs and the expression of certain genes. For the present study, we investigated the regulatory relationship between microRNA ‐222 (miR‐222) andTHBS1 in porcine follicular granulosa cells (pGCs) and its effects on apoptosis to provide empirical data for developing methods to improve pig fecundity. Results revealed that miR ‐222 promotes the proliferation of pGCs. MiRNA mimics and luciferase reporter assays revealed that miR‐222 functions as an anti‐apoptotic factor in pGCs. MiR‐222 mimics in pGCs result in the...
Source: Animal Science Journal - April 13, 2019 Category: Zoology Authors: Weihua Zhu, Min Yang, Jinnan Shang, Yiliang Xu, Yuanlang Wang, Qiangqiang Tao, Liang Zhang, Yueyun Ding, Yige Chen, Dongdong Zhao, Chonglong Wang, Mingxing Chu, Zongjun Yin, Xiaodong Zhang Tags: ORIGINAL ARTICLE Source Type: research

Leucine regulates slow ‐twitch muscle fibers expression and mitochondrial function by Sirt1/AMPK signaling in porcine skeletal muscle satellite cells
In this study, porcine skeletal muscle satellite cells cultured in differentiation medium were treated with 2 mM leucine for 3 days. Sirt1 inhibitor EX52 7, AMPK inhibitor compound C, and AMPKα1 siRNA were used to examine its underlying mechanism. Here we showed that leucine increased slow‐twitch muscle fibers and mitochondrial function‐related gene expression, as well as increased succinic dehydrogenase (SDH) and malate dehydrogenase (MDH) acti vities. Moreover, leucine increased the protein levels of Sirt1 and phospho‐AMPK. We also found that AMPKα1 siRNA, AMPK inhibitor compound C, or Sirt1 inhibitor EX527 atte...
Source: Animal Science Journal - December 6, 2018 Category: Zoology Authors: Xiaoling Chen, Lu Xiang, Gang Jia, Guangmang Liu, Hua Zhao, Zhiqing Huang Tags: ORIGINAL ARTICLE Source Type: research

Effects of silencing INH α gene by RNAi on the mRNA expressions of StAR, FST, INHβB, and FSHR genes in cultured sheep granulosa cells
Publication date: Available online 14 October 2017 Source:Small Ruminant Research Author(s): T. Li, A.T. Ma, Y.J. Zhang, Y.Q. Liu, S.Y. Wang, X.F. Shi, K. Gao, C.H. Duan The aim of the study was to investigate the effects of silencing the inhibin α-subunit (INHα) gene by RNAi on the mRNA levels of steroidogenic acute regulatory protein (StAR), follistatin (FST), inhibin β-subunit (INHβB), and FSH receptor (FSHR) in cultured sheep granulosa cells (GCs). The antral follicles, 3–7mm in diameter, were dissected from the ovaries of 15 Thin-tailed Han sheep (1-1.5 years old), and then GCs were isolated from antral follicl...
Source: Small Ruminant Research - October 24, 2017 Category: Zoology Source Type: research