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Use of deep sequencing to profile small RNAs derived from tomato spotted wilt orthotospovirus and hippeastrum chlorotic ringspot orthotospovirus in infected Capsicum annuum
Virus Res. 2021 Dec 12:198648. doi: 10.1016/j.virusres.2021.198648. Online ahead of print.ABSTRACTVirus-derived small RNAs are one of the key factors of RNA silencing in plant defence against viruses. We obtained virus-derived small interfering RNA profiles from Tomato spotted wilt orthotospovirus and Hippeastrum chlorotic ringspot orthotospovirus infected Capsicum annuum XX19 and XY11 by deep sequencing one day after inoculation. The vsiRNAs data were mapped to the TSWV and HCRV genomes, and the results showed that the vsiRNAs measured 19-24 nucleotides in length. Most of the vsiRNAs were mapped to the S segment of the vi...
Source: Virus Research - December 15, 2021 Category: Virology Authors: Xue Gao Zhi-Qiang Jia Hong-Zheng Tao Ye Xu Yong-Zhong Li Ya-Ting Liu Source Type: research

Differential response of diverse solanaceous hosts to tomato leaf curl New Delhi virus infection indicates coordinated action of NBS-LRR and RNAi-mediated host defense
Abstract Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus (family Geminiviridae) that infects a wide range of plants. ToLCNDV has emerged as an important pathogen and a serious threat to tomato production in India. A comparative and molecular analysis of ToLCNDV pathogenesis was performed on diverse solanaceous hosts (Capsicum annuum, Nicotiana benthamiana, N. tabacum, and Solanum lycopersicum). N. benthamiana was found to be the most susceptible host, whereas C. annuum showed resistance against an isolate of ToLCNDV collected in New Delhi from tomato (GenBank accession no. U15015 ...
Source: Archives of Virology - May 12, 2015 Category: Virology Source Type: research