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RNA interference in < i > Fasciola gigantica < /i > : Establishing and optimization of experimental RNAi in the newly excysted juveniles of the fluke
by Arun Anandanarayanan, Opinder Krishen Raina, Hniang Lalrinkima, Ajayta Rialch, Muthu Sankar, Anju Varghese Fasciolosis caused byFasciola gigantica is a neglected tropical disease but a constraint on the growth and productivity of cattle, buffaloes and sheep in the tropical countries of Asia and Africa. Resistance to commonly used anthelmintics inFasciola has increased the need to search for alternative therapeutic targets. RNA interference is the current tool of choice in the search for such targets inFasciola. The susceptibility of juvenileFasciola hepatica to double stranded (ds) RNA induced RNAi has been established...
Source: PLoS Neglected Tropical Diseases - December 12, 2017 Category: Tropical Medicine Authors: Arun Anandanarayanan Source Type: research

RNA interference in Fasciola hepatica newly excysted juveniles: Long dsRNA induces more persistent silencing than siRNA
Publication date: October 2014 Source:Molecular and Biochemical Parasitology, Volume 197, Issues 1–2 Author(s): Nicolás Dell’Oca , Tatiana Basika , Ileana Corvo , Estela Castillo , Paul J. Brindley , Gabriel Rinaldi , Jose F. Tort In trematodes RNA interference is the current tool of choice for functional analysis of genes since classical reverse genetic approaches remain unavailable. Whereas this approach has been optimized in schistosomes, few reports are available for other trematodes, likely reflecting the difficulties in the establishment of the technology. Here we standardized conditions for RNAi in the liver ...
Source: Molecular and Biochemical Parasitology - November 17, 2014 Category: Parasitology Source Type: research