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eIF5A is activated by virus infection or dsRNA and facilitates virus replication through modulation of interferon production
Active hypusine-modified initiation elongation factor 5A is critical for cell proliferation and differentiation, embryonic development, and innate immune response of macrophages to bacterial infection. Here, we demonstrate that both virus infection and double-stranded RNA viral mimic stimulation induce the hypusination of eIF5A. Furthermore, we show that activation of eIF5A is essential for the replication of several RNA viruses including influenza A virus, vesicular stomatitis virus, chikungunya virus, mayaro virus, una virus, zika virus, and punta toro virus. Finally, our data reveal that inhibition of eIF5A hypusination...
Source: Frontiers in cellular and infection microbiology - July 27, 2022 Category: Microbiology Source Type: research

Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses
Cell Host Microbe. 2021 Mar 29:S1931-3128(21)00132-3. doi: 10.1016/j.chom.2021.03.004. Online ahead of print.ABSTRACTArgonaute (AGO) proteins bind small RNAs to silence complementary RNA transcripts, and they are central to RNA interference (RNAi). RNAi is critical for regulation of gene expression and antiviral defense in Aedes aegypti mosquitoes, which transmit Zika, chikungunya, dengue, and yellow fever viruses. In mosquitoes, AGO1 mediates miRNA interactions, while AGO2 mediates siRNA interactions. We applied AGO-crosslinking immunoprecipitation (AGO-CLIP) for both AGO1 and AGO2, and we developed a universal software p...
Source: Cell Host and Microbe - April 1, 2021 Category: Microbiology Authors: Kathryn Rozen-Gagnon Meigang Gu Joseph M Luna Ji-Dung Luo Soon Yi Sasha Novack Eliana Jacobson Wei Wang Matthew R Paul Troels K H Scheel Thomas Carroll Charles M Rice Source Type: research