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Autophagy Is a Defense Mechanism Inhibiting Invasion and Inflammation During High-Virulent Haemophilus parasuis Infection in PK-15 Cells
In this study, we sought to investigate whether SH0165 (serovar 5, high-virulent strain) and HN0001 (serovar 6, non-virulent strain) infection induces autophagy and the specific role of autophagy in bacterial invasion and inflammation during H. parasuis infection. Moreover, we explored the mechanism underlying autophagy regulated inflammation through inflammatory signaling cascades during H. parasuis infection. This observation could provide useful information for further understanding the role of autophagy in H. parasuis infection and improve our knowledge of new strategies against this pathogen. Materials and Methods B...
Source: Frontiers in cellular and infection microbiology - April 15, 2019 Category: Microbiology Source Type: research

Interleukin 10 suppresses lysosome-mediated killing of Brucella abortus in cultured macrophages Immunology
In this study, we investigated the regulatory role of IL10 in the immune response to B. abortus infection. B. abortus–infected macrophages were treated with either IL10 siRNA or recombinant IL10 (rIL10), and the expression of phagolysosome- or inflammation-related genes was evaluated by qRT-PCR and Western blotting. Phagolysosome fusion was monitored by fluorescence microscopy. We found that the synthesis of several membrane-trafficking regulators and lysosomal enzymes was suppressed by IL10 during infection, resulting in a significant increase in the recruitment of hydrolytic enzymes by Brucella-containing phagosomes (B...
Source: Journal of Biological Chemistry - March 2, 2018 Category: Chemistry Authors: Huynh Tan Hop, Alisha Wehdnesday Bernardo Reyes, Tran Xuan Ngoc Huy, Lauren Togonon Arayan, WonGi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim Tags: Microbiology Source Type: research

Repetitive extragenic palindromic DNA sequences from Brucella  melitensis stimulate Toll-like receptor 9 signaling in macrophages.
Repetitive extragenic palindromic DNA sequences from Brucella melitensis stimulate Toll-like receptor 9 signaling in macrophages. Mol Med Rep. 2016 Dec 06;: Authors: Yu H, Bai L, Zhang Y, Wang Z, Yu Y Abstract Brucella DNA activates the host innate immune system via the intracellular Toll-like receptor 9 (TLR9). However, the Brucella DNA sequences which are responsible for these immunostimulatory effects remain to be elucidated. The present study demonstrated that repetitive extragenic palindromic (REPs) sequences present in Brucella DNA were able to stimulate macrophages through TLR9. The induction o...
Source: Molecular Medicine Reports - December 7, 2016 Category: Molecular Biology Tags: Mol Med Rep Source Type: research