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Tonic suppression of < i > PCAT29 < /i > by the IL-6 signaling pathway in prostate cancer: Reversal by resveratrol
In this study we focused on the lncRNA, prostate cancer associated transc ript 29 (PCAT29), a putative tumor suppressive gene. Our data show that the expression ofPCAT29 was reduced in prostate cancer tumors compared to paired perinormal prostate tissues. We also observed substantially lower levels ofPCAT29 in DU145 and LNCaP cells compared to normal prostate (RWPE-1) cells. IL-6, a cytokine which is elevated in prostate tumors, reduced the expression ofPCAT29 in both DU145 and LNCaP cells by activating signal transducer and activator of transcription 3 (STAT3). One downstream target of STAT3 is microRNA(miR)-21, inhibitio...
Source: PLoS One - May 3, 2017 Category: Biomedical Science Authors: Raheem F. H. Al Aameri Source Type: research

Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells
In this study, by using immunohistochemical staining, we found higher expr ession level of Med19 in PCa tissues than in adjacent benign prostate tissues. We then knocked down the Med19 expression in PCa cell lines LNCaP and PC3 by using lentivirus siRNA. Cell proliferation, anchor-independent growth, migration, and invasion were suppressed in Med19 knockdown PCa cells. In nude mice xenograft model, we found that Med19 knockdown PCa cells formed smaller tumors with lower proliferation index than did control cells. In the mechanism study, we found that Med19 could regulate genes involved in cell proliferation, cell cycle, an...
Source: PLoS One - January 25, 2017 Category: Biomedical Science Authors: Shengqiang Yu Source Type: research

The Anoikis Effector Bit1 Inhibits EMT through Attenuation of TLE1-Mediated Repression of E-Cadherin in Lung Cancer Cells
by Xin Yao, Tri Pham, Brandi Temple, Selena Gray, Cornita Cannon, Renwei Chen, Asim B. Abdel-Mageed, Hector Biliran The mitochondrial Bcl-2 inhibitor of transcription 1 (Bit1) protein is part of an anoikis-regulating pathway that is selectively dependent on integrins. We previously demonstrated that the caspase-independent apoptotic effector Bit1 exerts tumor suppressive function in lung cancer in part by inhib iting anoikis resistance and anchorage-independent growthin vitro and tumorigenicityin vivo. Herein we show a novel function of Bit1 as an inhibitor cell migration and epithelial –mesenchymal transition (EMT) in ...
Source: PLoS One - September 20, 2016 Category: Biomedical Science Authors: Xin Yao Source Type: research

Inhibitory Mechanism of FAT4 Gene Expression in Response to Actin Dynamics during Src-Induced Carcinogenesis
In this study, we show that transient activation of the Src oncoprotein represses FAT4 mRNA expression through actin depolymerization in the immortalized normal human mammary epithelial cell line MCF-10A. Src activation causes actin depolymerization via the MEK/Erk/Cofilin cascade. The MEK inhibitor U0126 blocks the inhibitory effect of Src on FAT4 mRNA expression and Src-induced actin depolymerization. To determine whether actin dynamics act on the regulation of FAT4 mRNA expression, we treated MCF-10A cells with the ROCK inhibitor Y-27632. Y-27632 treatment decreased FAT4 mRNA expression. This suppressive effect was bloc...
Source: PLoS One - February 13, 2015 Category: Biomedical Science Authors: Takao Ito et al. Source Type: research