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Dual inhibition of BDNF/TrkB and autophagy: a promising therapeutic approach for colorectal cancer
Abstract Colorectal cancer (CRC) is the most common digestive cancer in the Western world. Despite effective therapies, resistance and/or recurrence frequently occur. The present study investigated the impact of two survival pathways—neurotrophic factors (TrkB/BDNF) and autophagy—on cell fate and tumour evolution. In vitro studies were performed on two CRC cell lines, SW480 (primary tumour) and SW620 (lymph node invasion), which were also used for subcutaneous xenografts on a nude mouse model. In addition, the presence of neurotrophic factors (NTs) and autophagy markers were assessed in tissue samples representative of...
Source: Journal of Cellular and Molecular Medicine - June 9, 2017 Category: Molecular Biology Authors: Cl ément Mazouffre, Sophie Geyl, Aurélie Perraud, Sabrina Blondy, Marie‐Odile Jauberteau, Muriel Mathonnet, Mireille Verdier Tags: Original Article Source Type: research

Overexpression of deubiquitinating enzyme USP28 promoted non‐small cell lung cancer growth
Abstract Non‐small cell lung cancer (NSCLC) accounts for most lung cancer. To develop new therapy required the elucidation of NSCLC pathogenesis. The deubiquitinating enzymes USP 28 has been identified and studied in colon and breast carcinomas. However, the role of USP28 in NSCLC is unknown. The level mRNA or protein level of USP28 were measured by qRT‐PCR or immunohistochemistry (IHC). The role of USP28 in patient survival was revealed by Kaplan–Meier plot of overall survival in NSCLC patients. USP28 was up or down regulated by overexpression plasmid or siRNA transfection. Cell proliferation and apoptosis was assay...
Source: Journal of Cellular and Molecular Medicine - February 5, 2015 Category: Molecular Biology Authors: Lei Zhang, Biao Xu, Yong Qiang, Hairong Huang, Changtian Wang, Demin Li, Jianjun Qian Tags: Original Article Source Type: research

KITLG is a novel target of miR‐34c that is associated with the inhibition of growth and invasion in colorectal cancer cells
In conclusion, our results demonstrated that miR‐34c might interfere with KITLG‐related CRC and could be a novel molecular target for CRC patients.
Source: Journal of Cellular and Molecular Medicine - September 12, 2014 Category: Molecular Biology Authors: Shu Yang, Wen‐shuai Li, Fang Dong, Hai‐mei Sun, Bo Wu, Jun Tan, Wan‐jing Zou, De‐shan Zhou Tags: Original Article Source Type: research

Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway
This study aimed to demonstrate the effect of brucine on tumour angiogenesis and its mechanism of action. The anti‐angiogenic effect was evaluated on the chicken chorioallantoic membrane (CAM) model and tube formation. The mechanism was demonstrated through detecting mRNA and protein expressions of VEGFR2 (KDR), PKCα, PLCγ and Raf1 by reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blot (WB), as well as expressions of VEGF and PKCβ and mTOR by ELISA and WB. The results showed that brucine significantly reduced angiogenesis of CAM and tube formation, inhibited the VEGF secretion and mTOR expres...
Source: Journal of Cellular and Molecular Medicine - August 2, 2013 Category: Molecular Biology Authors: Wenjuan Luo, Xiaoli Wang, Lei Zheng, Yingzhuan Zhan, Dongdong Zhang, Jie Zhang, Yanmin Zhang Tags: Original Article Source Type: research