• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Increased Lipogenesis Is Critical for Self ‐Renewal and Growth of Breast Cancer Stem Cells: Impact of Omega‐3 Fatty Acids
Breast cancer stem cells (CSC) have a distinct fatty acid profile due to the aberrant expressions of lipogenic enzymes (eg, FAS, SCD1, and FADS1/2), which are highly involved in sustaining their self-renewal, and proliferative capabilities. Omega-3 PUFA can effectively suppress the self-renewal and growth of breast CSC by downregulation of the lipogenic enzymes, especially SCD1. AbstractAberrant lipid metabolism has recently been recognized as a new hallmark of malignancy, but the characteristics of fatty acid metabolism in breast cancer stem cells (BCSC) and potential interventions targeting this pathway remain to be addr...
Source: Stem Cells - September 6, 2021 Category: Stem Cells Authors: Haiqing Luo, Chih ‐Yu Chen, Xiangyong Li, Xin Zhang, Chien‐Wen Su, Yinhua Liu, Tinglan Cao, Lei Hao, Meng Wang, Jing Xuan Kang Tags: Cancer Stem Cells Source Type: research

Colorectal Cancer Stem Cells Acquire Chemoresistance through the Upregulation of FBXW7 and the Consequent Degradation of c ‐Myc
In conclusion, our study revealed that a CSC‐specific FBXW7‐regulatory mechanism is strongly associated with resistance to chemotherapeutic agents. Inhibition of FBXW7‐upregulation in CSCs following chemotherapy may enhance the response to anticancer agents and represents an attractive strategy for the elimination of colorectal CSCs. This article is protected by copyright. All rights reserved. LGR5(+) colorectal CSCs are proliferative and highly expressing c‐Myc with the absence of anticancer agents. During the cell cycle arrest caused by the anticancer agents, c‐Myc expression was substantially decreased in col...
Source: Stem Cells - July 11, 2017 Category: Stem Cells Authors: Daisuke Izumi, Takatsugu Ishimoto, Keisuke Miyake, Tsugio Eto, Kota Arima, Yuki Kiyozumi, Tomoyuki Uchihara, Junji Kurashige, Masaaki Iwatsuki, Yoshifumi Baba, Yasuo Sakamoto, Yuji Miyamoto, Naoya Yoshida, Masayuki Watanabe, Ajay Goel, Patrick Tan, Hideo Tags: Cancer Stem Cells Source Type: research

TGFβ‐Responsive HMOX1 Expression Is Associated with Stemness and Invasion in Glioblastoma Multiforme
Abstract Glioblastoma multiforme (GBM) is the most common and lethal adult brain tumor. Resistance to standard radiation and chemotherapy is thought to involve survival of GBM cancer stem cells (CSCs). To date, no single marker for identifying GBM CSCs has been able to capture the diversity of CSC populations, justifying the needs for additional CSC markers for better characterization. Employing targeted mass spectrometry, here we present five cell‐surface markers HMOX1, SLC16A1, CADM1, SCAMP3, and CLCC1 which were found to be elevated in CSCs relative to healthy neural stem cells (NSCs). Transcriptomic analyses of REMBR...
Source: Stem Cells - July 3, 2016 Category: Stem Cells Authors: Dhiman Ghosh, Ilya V. Ulasov, LiPing Chen, Lualhati E. Harkins, Karolina Wallenborg, Parvinder Hothi, Steven Rostad, Leroy Hood, Charles S. Cobbs Tags: Cancer Stem Cell Source Type: research

TGF β‐Responsive HMOX1 Expression Is Associated with Stemness and Invasion in Glioblastoma Multiforme
Abstract Glioblastoma multiforme (GBM) is the most common and lethal adult brain tumor. Resistance to standard radiation and chemotherapy is thought to involve survival of GBM cancer stem cells (CSCs). To date, no single marker for identifying GBM CSCs has been able to capture the diversity of CSC populations, justifying the needs for additional CSC markers for better characterization. Employing targeted mass spectrometry, here we present five cell‐surface markers HMOX1, SLC16A1, CADM1, SCAMP3, and CLCC1 which were found to be elevated in CSCs relative to healthy neural stem cells (NSCs). Transcriptomic analyses of REMBR...
Source: Stem Cells - July 3, 2016 Category: Stem Cells Authors: Dhiman Ghosh, Ilya V. Ulasov, LiPing Chen, Lualhati E. Harkins, Karolina Wallenborg, Parvinder Hothi, Steven Rostad, Leroy Hood, Charles S. Cobbs Tags: Cancer Stem Cell Source Type: research

TGFβ‐Responsive HMOX1 Expression is Associated with Stemness and Invasion in GBM
This article is protected by copyright. All rights reserved. Targeted proteomic analyses revealed elevated expressions of transmembrane proteins HMOX1 and SLC16A1 on the surface of glioblastoma cancer stem cells (CSCs) relative to healthy neural stem cells (NSCs). In the hypoxic region of the tumor, these proteins were found to be expressed among pseudopalisading glioma cells that also express known stem cell factors. From biological assays that are known to evaluate stemness, HMOX1 expression was found to be associated with stemness that could be regulated through TGFβ and PTEN signaling networks. Additionally, siRNA me...
Source: Stem Cells - June 28, 2016 Category: Stem Cells Authors: Dhiman Ghosh, Ilya V. Ulasov, LiPing Chen, Karolina Wallenborg, Parvinder Hothi, Leroy Hood, Charles S. Cobbs Tags: Cancer Stem Cells Source Type: research

The PI3K/AKT/c ‐MYC Axis Promotes the Acquisition of Cancer Stem‐Like Features in Esophageal Squamous Cell Carcinoma
In conclusion, we have described a novel intratumoral heterogeneity in ESCC. The identification of the PI3K/AKT/c‐MYC axis as a driver of CSC‐like features carries therapeutic implications. Stem Cells 2016;34:2040–2051 Oxidative stress induced by H2O2 promotes RU/RR conversion and the acquisition of CSC‐like properties. (A) Reactive oxygen species (ROS) induced by H2O2 (500 μM for 24 hours) was measured by flow cytometry. (B) H2O2‐induced RU/RR conversion in KYSE150‐RU cells, as indicated by the increase of GFP‐positive cells and luciferase activity. Representative flow cytometry data at Day 4 was shown in...
Source: Stem Cells - May 22, 2016 Category: Stem Cells Authors: Hai ‐Feng Zhang, Chengsheng Wu, Abdulraheem Alshareef, Nidhi Gupta, Qing Zhao, Xiu‐E Xu, Ji‐Wei Jiao, En‐Min Li, Li‐Yan Xu, Raymond Lai Tags: Cancer Stem Cells Source Type: research

The PI3K/AKT/c‐MYC axis promotes the acquisition of cancer stem‐like features in esophageal squamous cell carcinoma
In conclusion, we have described a novel intra‐tumoral heterogeneity in ESCC. The identification of the PI3K/AKT/c‐MYC axis as a driver of CSC‐like features carries therapeutic implications. This article is protected by copyright. All rights reserved. Oxidative stress induced by H2O2 promotes RU/RR conversion and the acquisition of CSC‐like properties. (A) Reactive oxygen species (ROS) induced by H2O2 (500μM for 24h) was measured by flow cytometry. (B) H2O2‐induced RU/RR conversion in KYSE150‐RU cells, as indicated by the increase of GFP‐positive cells and luciferase activity. Representative flow cytometry ...
Source: Stem Cells - March 31, 2016 Category: Stem Cells Authors: Hai‐Feng Zhang, Chengsheng Wu, Abdulraheem Alshareef, Nidhi Gupta, Qing Zhao, Xiu‐E Xu, Ji‐Wei Jiao, En‐Min Li, Li‐Yan Xu, Raymond Lai Tags: Cancer Stem Cells Source Type: research

Peroxiredoxin II is essential for maintaining stemness by redox regulation in liver cancer cells
This article is protected by copyright. All rights reserved.
Source: Stem Cells - February 11, 2016 Category: Stem Cells Authors: Taeho Kwon, Yesol Bak, Young‐Ho Park, Gyu‐Beom Jang, Jeong‐Seok Nam, Jeong Eun Yoo, Young Nyun Park, In Seon Bak, Jin‐Man Kim, Do‐Young Yoon, Dae‐Yeul Yu Tags: Cancer Stem Cells Source Type: research

Geminin Is Essential to Prevent DNA Re‐Replication‐Dependent Apoptosis in Pluripotent Cells, but not in Differentiated Cells
Abstract Geminin is a dual‐function protein unique to multicellular animals with roles in modulating gene expression and preventing DNA re‐replication. Here, we show that geminin is essential at the beginning of mammalian development to prevent DNA re‐replication in pluripotent cells, exemplified by embryonic stem cells, as they undergo self‐renewal and differentiation. Embryonic stem cells, embryonic fibroblasts, and immortalized fibroblasts were characterized before and after geminin was depleted either by gene ablation or siRNA. Depletion of geminin under conditions that promote either self‐renewal or differen...
Source: Stem Cells - July 16, 2015 Category: Stem Cells Authors: Yi‐Yuan Huang, Kotaro J. Kaneko, Haiyan Pan, Melvin L. DePamphilis Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Geminin Is Essential To Prevent DNA Re‐Replication Dependent Apoptosis In Pluripotent Cells, But Not In Differentiated Cells
This article is protected by copyright. All rights reserved.
Source: Stem Cells - July 1, 2015 Category: Stem Cells Authors: Yi‐Yuan Huang, Kotaro J. Kaneko, Haiyan Pan, Melvin L. DePamphilis Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Focal Adhesion Kinase and Wnt Signaling Regulate Human Ductal Carcinoma In Situ Stem Cell Activity and Response to Radiotherapy
Abstract Cancer stem cells (CSCs) can avoid or efficiently repair DNA damage from radio and chemotherapy, which suggests they play a role in disease recurrence. Twenty percentage of patients treated with surgery and radiotherapy for ductal carcinoma in situ (DCIS) of the breast recur and our previous data show that high grade DCIS have increased numbers of CSCs. Here, we investigate the role of focal adhesion kinase (FAK) and Wnt pathways in DCIS stem cells and their capacity to survive irradiation. Using DCIS cell lines and patient samples, we demonstrate that CSC‐enriched populations are relatively radioresistant and p...
Source: Stem Cells - January 22, 2015 Category: Stem Cells Authors: Kathryn E. Williams, Nigel J. Bundred, Göran Landberg, Robert B. Clarke, Gillian Farnie Tags: Cancer Stem Cells Source Type: research

Focal adhesion kinase and wnt signalling regulates human ductal carcinoma in situ stem cell activity and response to radiotherapy
Abstract Cancer stem cells (CSCs) can avoid or efficiently repair DNA damage from radio and chemotherapy, which suggests they play a role in disease recurrence. 20% of patients treated with surgery and radiotherapy for ductal carcinoma in Situ (DCIS) of the breast recur and our previous data shows that high grade DCIS have increased numbers of CSCs. Here, we investigate the role of Focal Adhesion Kinase (FAK) and Wnt pathways in DCIS stem cells and their capacity to survive irradiation. Using DCIS cell lines and patient samples we demonstrate that CSC‐enriched populations are relatively radioresistant and possess high FA...
Source: Stem Cells - September 3, 2014 Category: Stem Cells Authors: Kathryn E Williams, Nigel J Bundred, Göran Landberg, Robert B Clarke, Gillian Farnie Tags: Original Research Source Type: research