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Inactivation of histone deacetylase 1 (hdac1) but not hdac2 is required for the glucocorticoid-dependent ccaat/enhancer binding protein α (c/ebpα) expression and preadipocyte differentiation.
In this study, we sought to demonstrate using two different strategies the definite role of HDACl in adipogenesis. By using siRNA-mediated knockdown of HDAC1 and by generating an enzymatically inactive HDAC1D181A by site-directed mutagenesis, we were able to show that HDAC1, but not HDAC2, suppresses GR-potentiated preadipocyte differentiation by decreasing C/ebpα and Pparγ expression levels at the onset of differentiation. Finally, we demonstrate that HDAC1D181A acts as a dominant negative mutant of HDACl during adipogenesis by modulating C/EBPβ transcriptional activity on the C/ebpα promoter. PMID: 25203139 [Pub...
Source: Endocrinology - September 9, 2014 Category: Endocrinology Authors: Kuzmochka C, Abdou HS, Haché RJ, Atlas E Tags: Endocrinology Source Type: research